产丙酮酸棒状杆菌的鉴定及微生物学性状研究

发布时间：2019-04-16 13:41

【摘要】：目的对临床分离于皮脂腺囊肿标本中的菌株进行表型和基因型鉴定,描述该菌株的生物学特性及致病性,为临床诊断提供准确地病原学依据。方法对分离菌株进行革兰氏染色等,并使用VITEK 2Compact全自动微生物分析仪进行鉴定。采用K-B法进行药敏试验。提取分离菌株DNA,采用通用引物对16S rRNA基因进行PCR扩增并测序,将测序结果与GenBank中收录的16S rRNA基因序列进行BLAST同源性对比。结果分离菌株经VITEK 2Compact鉴定为玫瑰色库克菌,后经16S rRNA序列测定方法鉴定,分离菌株为产丙酮酸棒状杆菌。药敏试验显示该菌株对四环素、万古霉素、利福平敏感,对青霉素、红霉素、克林霉素、环丙沙星、左氧氟沙星、复方新诺明、头孢吡肟、亚胺培南、庆大霉素等均耐药。结论对于表现型不易鉴定或鉴定不准确的细菌,采用16SrRNA序列测定的方法进行鉴定是最准确的。产丙酮酸棒状杆菌是引起患者疾病的致病菌。在完善产丙酮酸棒状杆菌生化反应信息的同时,探索出有效可行的生物学鉴定方法。
[Abstract]:Aim to identify the phenotypes and genotypes of the strains isolated from sebaceous gland cyst specimens and to describe the biological characteristics and pathogenicity of the strains in order to provide accurate etiological basis for clinical diagnosis. Methods the isolated strains were stained by Gram and identified by VITEK 2Compact automatic microbiological analyzer. K B method was used for drug sensitivity test. The 16s rRNA gene was amplified and sequenced by PCR with universal primers from the isolated strain DNA,. The BLAST homology of the 16s rRNA gene was compared with that of the 16s rRNA gene included in GenBank. Results the isolated strain was identified as Corynebacterium roseus by VITEK 2Compact and then identified by 16s rRNA sequencing. The isolated strain was Corynebacterium pyruvate-producing. The susceptibility test showed that the strain was sensitive to tetracycline, vancomycin, rifampicin, and resistant to penicillin, erythromycin, clindamycin, ciprofloxacin, levofloxacin, compound sinomine, cefepime, imipenem and gentamicin. Conclusion 16SrRNA sequencing is the most accurate method for identification of bacteria which are not easy to be identified or not accurately identified. Corynebacterium pyruvate-producing bacteria is the cause of disease in patients. In order to improve the biochemical reaction information of Corynebacterium pyruvate-producing bacteria, an effective and feasible biological identification method was explored.
【作者单位】：潍坊医学院医学检验学系纳米医学技术研究所;潍坊医学院附属医院检验科;
【分类号】：R446.5

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