极化液对内毒素休克兔脑保护作用的研究

发布时间：2019-04-19 19:38

【摘要】：目的:研究极化液对内毒素休克兔脑保护作用并探讨其可能机制。方法:30只新西兰大白兔,随机分为正常对照组(NS组)、内毒素休克组(ES组)、极化液组(GIK组),每组10只。NS组静脉泵注复方氯化钠(5ml/kg.h),ES组和GIK组静脉注射LPS 0.5mg/kg建立内毒素休克模型后分别泵注复方氯化钠和GIK(5ml/kg.h共6小时)干预治疗。测定各时间点生命体征(平均动脉压、呼吸频率、体温)、血糖、血气分析指标(PH、PaCO_2、血钾、乳酸),ELISA法检测血清NSE浓度。实验结束后立即处死白兔取脑组织,左脑皮质制备10%组织匀浆,检测氧化指标(H2O_2、NO、iNOS、MDA)、抗氧化指标(SOD、T-AOC)、Na~+-K~+-ATP酶和Ca~(2+)-Mg~(2+)-ATP酶活力,称量剩余脑组织干湿重并计算含水量。光镜和电镜下观察右脑皮层形态学改变。结果:1.与NS组相比,实验兔注射LPS后平均动脉压、PH和PaCO_2明显降低(P㩳0.05),呼吸增快,乳酸和体温显著升高(P㩳0.05)。ES组和GIK组治疗过程中平均动脉压和乳酸均逐渐升高。GIK组与ES组相比,平均动脉压升高更显著(P㩳0.05),乳酸明显降低(P㩳0.05),其余指标没有统计学差异(P0.05)。2.相对于NS组,ES组和GIK组脑组织匀浆中氧化指标、血清中NSE浓度、脑含水量均显著升高(P㩳0.05),匀浆中抗氧化指标、Na~+-K~+-ATP酶和Ca~(2+)-Mg~(2+)-ATP酶活性均明显降低(P㩳0.05)。而与ES组比较,GIK组氧化指标、NSE浓度、脑含水量均明显降低(P㩳0.05),抗氧化指标、Na~+-K~+-ATP酶和Ca~(2+)-Mg~(2+)-ATP酶活力均显著升高(P㩳0.05)。3.病理观察NS组未见异常,ES组和GIK组脑细胞和线粒体损伤明显,但GIK组病理损伤轻于ES组。结论:极化液对内毒素休克兔脑损伤有保护作用,可能与其改善血流动力学,降低氧化应激水平,减轻线粒体损伤有关。
[Abstract]:Objective: to study the protective effect of polarizing liquid on the brain of rabbits with endotoxic shock and to explore its possible mechanism. Methods: thirty New Zealand white rabbits were randomly divided into three groups: normal control group (NS group), endotoxin shock group (ES group), polarized solution group (GIK group), 10 rabbits in each group. NS group received intravenous infusion of compound sodium chloride (5ml/kg.h). The endotoxic shock model was established by intravenous injection of LPS 0.5mg/kg in ES group and GIK group, and then was treated with compound sodium chloride and GIK (5ml/kg.h for 6 hours). The vital signs (mean arterial pressure, respiratory rate, body temperature), blood glucose and blood gas analysis indexes (PH,PaCO_2, blood potassium, lactic acid), ELISA method) were measured at each time point. Serum NSE concentration was measured by lactic acid), ELISA method. At the end of the experiment, the white rabbits were killed and 10% tissue homogenate was prepared in the left cerebral cortex. The oxidation indexes (H 2O 2, no, iNOS, MDA), antioxidant index (SOD,T-AOC) were measured. The activities of Na~-K- ATP enzyme and Ca~ (2)-Mg~ (2)-ATP enzyme were measured, the dry and wet weight of the remaining brain tissue was measured and the water content was calculated. Morphological changes of right cerebral cortex were observed under light and electron microscope. Results: 1. Compared with NS group, the mean arterial pressure (map), PH and PaCO_2 (P < 0.05) and respiratory rate (P < 0.05) were significantly decreased after LPS injection in the experimental rabbits. The mean arterial pressure and lactic acid increased gradually during the treatment in). ES group and GIK group, and the mean arterial pressure increased more significantly in GIK group than that in ES group (P0. 05), while the mean arterial pressure and lactic acid in GIK group were significantly higher than those in ES group (P < 0. 05). Lactic acid decreased significantly (P < 0.05), but there was no statistical difference between the other indexes (P0.05). Compared with NS group, ES group and GIK group, the oxidative index in brain homogenate, serum NSE concentration and brain water content were significantly increased (P < 0.05), and the antioxidant index in homogenate was significantly higher than that in GIK group (P < 0.05). The activities of Na~-K- ATP enzyme and Ca~ (2)-Mg~ (2)-ATP enzyme were significantly decreased (P0. 05). Compared with ES group, the oxidative index, NSE concentration and brain water content in GIK group were significantly decreased, while the antioxidant index, Na~-K3. ATP enzyme and Ca~ (2)-Mg~ (2)-ATP enzyme activity were significantly increased (P < 0. 05). No abnormality was observed in NS group. The damage of brain cells and mitochondria in ES group and GIK group was obvious, but the pathological damage in GIK group was lighter than that in ES group. Conclusion: polarizing solution has protective effect on brain injury in rabbits with endotoxic shock, which may be related to the improvement of hemodynamics, reduction of oxidative stress level and reduction of mitochondrial damage.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R459.7

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