酶联免疫吸附分析双抗夹心法检测临床鼻咽抽吸物中呼吸道合胞病毒

发布时间：2019-06-27 20:41

【摘要】：呼吸道合胞病毒(respiratory syncytial virus,RSV)是引起5周岁以下儿童下呼吸道感染的主要病原体.目前常用的试剂盒是美国Diagnostic Hybrids公司研发的7项呼吸道病毒检测试剂盒,但其价格昂贵,且需荧光显微镜辅助诊断,不利于在基层医疗机构中推广,国内尚无上市的快速诊断试剂用于临床检测.为此建立用于RSV抗原检测的早期快速诊断试剂,并以反转录聚合酶链式反应(RT-PCR)检测为参比,同时使用酶联免疫吸附分析(ELISA)双抗夹心法和免疫荧光法对112份临床鼻咽抽吸物(NPA)样本进行检测.ELISA双抗夹心法和免疫荧光法的灵敏度分别为79.31%和74.14%,特异度分别为100%和96.30%,Kappa值分别为0.787 1和0.689 5,两者均与RT-PCR检测结果具有高度一致性.此外,将ELISA双抗夹心法与免疫荧光法比较,两者无显著差异(p0.05).综上,ELISA双抗夹心法操作简便,特异性高,有望代替进口检测试剂盒完成RSV感染的早期快速诊断.
[Abstract]:Respiratory syncytial virus (respiratory syncytial virus,RSV) is the main pathogen of lower respiratory tract infection in children under 5 years of age. At present, the commonly used kit is seven respiratory virus detection kits developed by Diagnostic Hybrids Company of the United States, but it is expensive and needs fluorescence microscope to assist diagnosis, which is not conducive to promotion in primary medical institutions. There is no rapid diagnostic reagent on the market in China for clinical detection. In this paper, an early rapid diagnostic reagent for RSV antigen detection was established and compared with reverse transcriptional polymerase chain reaction (RT-PCR). At the same time, (ELISA) double antibody sandwich method and immunofluorescence method were used to detect (NPA) samples of clinical nasopharyngeal aspiration. The sensitivity of Elisa double antibody sandwich method and immunofluorescence method were 79.31% and 74.14%, respectively. The specificity was 100% and 96.30%, and the Kappa value was 0.787 1 and 0.689 5, respectively. Both of them were in good agreement with the results of RT-PCR. In addition, there was no significant difference between ELISA double antibody sandwich method and immunofluorescence method (p0.05). In summary, ELISA double antibody sandwich method is simple and specific, and is expected to replace the imported detection kit to complete the early and rapid diagnosis of RSV infection.
【作者单位】：厦门大学分子疫苗学与分子诊断学国家重点实验室;厦门大学公共卫生学院国家传染病诊断试剂与疫苗工程技术研究中心;
【基金】：国家自然科学基金(81401668) NSFC-NIH生物医学合作研究项目(812111615) 福建省科技创新平台建设计划项目(2014Y2101)
【分类号】：R446.6

【参考文献】