凝胶卡血型鉴定正反不一致的原因分析

发布时间：2019-07-19 06:40

【摘要】：目的:探讨凝胶卡法鉴定ABO血型正反定型不一致的原因及其疾病分布的影响因素,并提出解决方法。方法:收集凝胶卡初检血型正反定型不一致血液标本240例,送血型参比实验室做经典试管法及唾液血型物质等血清学实验,在血清学方法无法确定时进行基因分型方法试验。结果:在ABO血型正反定型初检不一致标本240例中,假凝集采用试管法后正反定型一致107例(44.58%),不一致的133例(55.42%),其中冷凝集素35例(14.58%)、AB抗原性减弱22例(9.17%)、ABO亚型16例(6.67%)、直抗阳性12例(5.00%)、抗体缺失或减弱11例(4.58%)、因药物及蛋白引起假凝集11例(4.58%)、唾液血型物质11例(4.58%)、ABO血型以外的同种抗体8例(3.33%)、异基因骨髓移植7例(2.92%);疾病分布情况为:血液病(16.83%)、肿瘤(11.88%)、心肺疾病(11.39%),经卡方适度检验分析,疾病分布差异显著。结论:ABO血型正反定型不一致有多种因素影响,其中凝胶卡血型鉴定假阳性较多;在血液病、肿瘤、心肺疾病等方面高发,应予以足够的重视。
[Abstract]:Objective: to investigate the causes of inconsistency of positive and negative typing of ABO blood group by gel card method and the influencing factors of disease distribution, and to put forward some solutions. Methods: 240 blood samples with inconsistent blood group positive and negative typing were collected. The classical test tube method and saliva blood group substance serological test were carried out in the blood group reference laboratory. When the serological method could not be determined, the genotyping test was carried out. Results: among 240 cases of inconsistency of positive and negative typing of ABO blood group, 107 cases (44.58%) were positive and negative, 133 cases (55.42%) were inconsistent, including 35 cases (14.58%) of), AB antigenicity weakened (9.17%), ABO subgroup 16 cases (6.67%), 12 cases (5.00%) of direct antibody positive, 11 cases (4.58%) of antibody deletion or weakening. False aggregation caused by drugs and proteins was found in 11 cases (4.58%), saliva blood group substances in 11 cases (4.58%) other than), ABO blood group in 8 cases (3.33%), allogenic bone marrow transplantation in 7 cases (2.92%). The distribution of diseases was as follows: hematological diseases (16.83%), tumors (11.88%) and cardiopulmonary diseases (11.39%). The distribution of diseases was significantly different by chi-square moderate test. Conclusion: there are many factors affecting the inconsistency of positive and negative typing of ABO blood group, among which the false positive rate of gel card blood group identification is more, and the high incidence of blood disease, tumor, cardiopulmonary disease and so on should be paid enough attention to.
【作者单位】：空军总医院输血科;成都军区总医院输血科;
【分类号】：R457.11

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