赤皮青冈种子低萌发率的转录组与蛋白质组分析

发布时间：2023-02-01 14:25

　　赤皮青冈（Cyclobalnopsis gilva）属于壳斗科植物,原产于中国和日本等东南亚国家。赤皮青冈是一种优良的硬木树种,但是其种子发芽率低和萌发整齐度较差。应用RNA-seq、2-DE和Label-free等方法探讨在mRNA和蛋白水平上赤皮青冈种子萌发的分子生理机制。应用Illumina HiSeq 2500平台对赤皮青冈种子的转录组进行分析。通过匹配Nr、SwissProt、GO和KEEG等公共数据库上序列,结果显示54,130条unigene被注释。应用GO和KEGG pathway数据库分析这些unigene,结果发现许多unigene富集于淀粉和蔗糖代谢、糖酵解、植物激素信号转导、ABC转运子、碳水化合物代谢、翻译后修饰以及核糖体结构与生物合成等生物学过程。这些转录组数据表明许多种子萌发基因可以作为参考基因数据库用于分析赤皮青冈种子萌发过程基因的差异表达。萌发种子与未萌发种子间的差异基因的分析结果显示26,003条unigenes被鉴定出在两样本间表达差异达2倍以上,其中在萌发种子中有9683条unigenes上调表达,16,320条unigenes下调表达。未萌发种... 

【文章页数】：117 页

【学位级别】：博士

【文章目录】：
Abbreviations
中文摘要
ABSTRACT
1 Introduction
    1.1 Physiological features in seed germination
    1.2 Transcriptomic approach to address seed germination
        1.2.1 Energy production for germination
        1.2.2 Genes involve in starch and sucrose metabolism
        1.2.3 Genes involve in protein mobilization
        1.2.4 Activation of developmental genes
        1.2.5 Defense genes activation
        1.2.6 Phytohormones in seed germination
    1.3 Proteomic approach to address seed germination
        1.3.1 Proteins involved in regulating germination
        1.3.2 Progress in proteomics related to seed germination
        1.3.3 Proteins involved in seed germination
    1.4 Transcriptome and proteomics interaction
        1.4.1 Transcription and translation activation
        1.4.2 Translation role in seed germination
        1.4.3 Comparison of the seed transcriptome and proteome
        1.4.4 Post-translational control of seed germination
    1.5 Objectives and technical route
        1.5.1 Objectives
        1.5.2 Technical route
2 Transcriptome analysis in Cyclobalnopsis gilva seed germination
    2.1 Materials and methods
        2.1.1 Plant materials
        2.1.2 RNA extraction and construction of cDNA libraries for transcriptomesequencing
        2.1.3 Illumina sequencing, assembly and annotation
        2.1.4 Analysis of quantitative real-time PCR (qRT-PCR)
        2.1.5 Analysis of physiological parameters
        2.1.6 Statistical analysis
    2.2 Results
        2.2.1 Sequence analysis and assembly from C. gilva seeds
        2.2.2 Analysis of differentially expressed unigenes (DEGs) by RNA-seq
        2.2.3 DEGs validation during seed germination
        2.2.4 Physiological profiles during seed germination
    2.3 Discussion
        2.3.1 Transcriptional responses in the glycolysis and TCA cycle pathways
        2.3.2 Transcriptional responses in starch and sucrose metabolism
3 Proteomic study on Cyclobalnopsis gilva seed germination
    3.1 Proteomic 2-DE approach to address low germination rate in C. gilva seeds
        3.1.1 Materials and methods
        3.1.2 Results
        3.1.3 Discussion
    3.2 Label-Free quantitative proteomics analysis in C. gilva seed germination
        3.2.1 Materials and methods
        3.2.2 Results
        3.2.3 Discussion
4 Conclusion
Reference
Publication
Acknowledgement


【参考文献】：
期刊论文
[1]Gene Expression Profile Changes in Germinating Rice[J]. Dongli He1,Chao Han1,2 and Pingfang Yang1 1Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China 2Graduate University of Chinese Academy of Sciences,Beijing 100049,China.  Journal of Integrative Plant Biology. 2011(10)



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