甜椒内质网小分子热激蛋白基因（CaHSP22.5）克隆及其在转基因烟草中的表达分析（英文）
发布时间：2021-04-22 00:54
【目的】克隆甜椒内质网小分子热激蛋白基因（CaHSP22.5）,并检测其表达特性,为探究其在植物抗逆中的调控机制提供理论依据。【方法】克隆CaHSP22.5基因并构建其表达载体pBI121-CaHSP22.5,转化农杆菌LBA4404后通过叶盘法转化野生型烟草,即获得CaHSP22.5转基因株系;以转pBI121空载体的野生型烟草为对照组。对转基因株系和野生型烟草进行4℃6 h低温处理,根据幼苗成活率测定CaHSP22.5基因表达对植株耐寒性的影响。采用脉冲振幅调制叶室荧光仪Li-6400测量4℃处理10 h后野生型植株与CaHSP22.5转基因株系叶绿素荧光,根据非光化学淬灭系数（NPQ）值测定CaHSP22.5基因表达对在低温下植株光合作用的影响;使用过氧化氢（H2O2）钛络合物法对烟草叶片中H2O2进行定量分析,通过测定活性氧（ROS）积累程度分析CaHSP22.5对植物光合系统的影响;采用检测柠檬酸合成酶（CS）活性的方法,在体外通过对变性CS复性的影响测定CaHSP22.5的分子伴侣活性。【结果】经低温处理后发现CaHSP22.5转基因株系13号、24号和32号幼苗的平均存活...
【文章来源】：南方农业学报. 2020,51(05)北大核心CSCD
【文章页数】：11 页
【文章目录】：
0 Introduction
1 Materials and methods
1.1 Plant materials and growth conditions
1.2 Isolation and sequencing of CaHSP22.5 gene from sweet pepper
1.3 Vector construction and plant transformation
1.4 Northern and western blotting analysis
1.5 Seed germination
1.6 Fluorescence measurements
1.7 Analysis of H2O2and O
1.8 Molecular chaperone activity assays
1.9 Statistical analysis
2 Results and analysis
2.1 Isolation and characterization of c DNA of Ca HSP22.5 gene from sweet pepper
2.2 Expression of Ca HSP22.5 gene in sweet pepper during heat stress
2.3 Enhanced chilling tolerance of transgenic tobacco over-expressing Ca HSP22.5 gene
2.4 Enhancedphotosynthesisoftransgenictobacco overexpressing Ca HSP22.5 gene under chilling stress
2.5 Reduced reactive oxygen species accumulation and alleviated lipid peroxidation in transgenic tobacco over-expressing Ca HSP22.5 gene under chilling stress
2.6 Molecular chaperone activity of Ca HSP22.5in vitro
3 Discussion
4 Conclusion
本文编号：3152837
【文章来源】：南方农业学报. 2020,51(05)北大核心CSCD
【文章页数】：11 页
【文章目录】：
0 Introduction
1 Materials and methods
1.1 Plant materials and growth conditions
1.2 Isolation and sequencing of CaHSP22.5 gene from sweet pepper
1.3 Vector construction and plant transformation
1.4 Northern and western blotting analysis
1.5 Seed germination
1.6 Fluorescence measurements
1.7 Analysis of H2O2and O
1.8 Molecular chaperone activity assays
1.9 Statistical analysis
2 Results and analysis
2.1 Isolation and characterization of c DNA of Ca HSP22.5 gene from sweet pepper
2.2 Expression of Ca HSP22.5 gene in sweet pepper during heat stress
2.3 Enhanced chilling tolerance of transgenic tobacco over-expressing Ca HSP22.5 gene
2.4 Enhancedphotosynthesisoftransgenictobacco overexpressing Ca HSP22.5 gene under chilling stress
2.5 Reduced reactive oxygen species accumulation and alleviated lipid peroxidation in transgenic tobacco over-expressing Ca HSP22.5 gene under chilling stress
2.6 Molecular chaperone activity of Ca HSP22.5in vitro
3 Discussion
4 Conclusion
本文编号：3152837
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