水分处理对黄瓜生理、品质及苦味相关基因表达的影响
发布时间：2021-06-17 03:55
黄瓜是重要的农业经济作物,黄瓜果实中含有非常丰富的营养物质,对人体具有非常高的价值。但是在农业生产中,由于栽培环境的影响,对黄瓜的生长,果实的品质等会带来影响,导致黄瓜果实苦味物质葫芦素C的形成等,从而进一步造成经济损失。温度、光照、养分、水分等通常是是栽培环境中的主要的影响因子。本试验选用中农16和黄山老黄瓜两个品种作为试验材料,将种子播种于穴盘中,等幼苗长到四叶一心时,定植于花盆中,进行统一管理为进一步的盆栽试验提供基础。等到黄瓜进入开花期并且根瓜坐住以后,统一进行不同水分的处理,水分处理主要包括1.8L/2d、1.5L/2d、1.2L/2d、0.9L/2d、0.6L/2d和0.3L/2d,1.8L/2d表示每两天浇足1.8L水,以此类推。每个品种的每个处理有5个重复。处理的过程中对处理5d和15d时,叶片的抗氧化酶活性和丙二醛进行检测;黄瓜果实进入成熟期,对黄瓜的产量、含水量、可溶性糖、可溶性蛋白、维生素C、有机酸和葫芦素C进行检测;对黄瓜植株是否为苦味类型进行基因鉴定同时对控制葫芦素C合成的Csa680基因进行RT-PCR。以研究黄瓜在结果期不同水分的处理与黄瓜叶片抗氧化酶活性...
【文章来源】：安徽农业大学安徽省
【文章页数】：38 页
【学位级别】：硕士
【部分图文】：
葫芦素的生物合成途径Fig.1ThebiosyntheticroutetoCucurbitacin
注：a 为黄山老黄瓜，b 为中农 16图 2 不同水分处理下黄瓜叶片在 5d 和 15d POD 活性的变化Fig.2 The variety of cucumber leaves POD under different water treatments in fifth and fifteenth days表 1 不同水分处理下黄瓜叶片在 5d 和 15d POD 活性Table 1 POD activity of cucumber leaves under different water treatments in fifth and fifteenth days黄山老黄瓜 中农 165d 15d 5d 15d1.8L/2d 1176.67±42.49eE 1201.67±51.37cC 1265.02±32.50eE 1269.04±49.81dD1.5L/2d 1194.33±134.80eE 1251.01±88.98cC 1312.01±32.17eE 1338.67±61.15dD1.2L/2d 1760.33±48.94dD 1840.33±69.82bB 2148.33±156.01dD 2548.34±60.60bB0.9L/2d 2346.00±65.91cC 2546.02±90.61aA 2726.03±52.64cC 3189.67±99.43aA0.6L/2d 3080.33±179.35bB 1947.01±79.66bB 3343.67±100.90bB 2465.34±38.00bB0.3L/2d 3510.33±84.70aA 1909.67±99.34bB 3997.11±147.80aA 2225.68±76.56cC注：同一列不同的小写字母数值间差异显著（P＜0.05），同一列不同大写字母数值间差异显著（P＜0.01）Note：There were significant differences between the different capital values of the same column (P <0.01)and there wasa significant difference between the lowercase letters in the same column (P <0.05)
注：a 为黄山老黄瓜，b 为中农 16图 3 不同水分处理下黄瓜叶片在 5d 和 15d SOD 活性的变化Fig.3 The variety of cucumber leaves SOD under different water treatments in fifth and fifteenth days表 2 不同水分处理下黄瓜叶片在 5d 和 15d SOD 活性Table 2 SOD activity of cucumber leaves under different water treatments in fifth and fifteenth days黄山老黄瓜 中农 165d 15d 5d 15d1.8L/2d 894.66±59.14dD 736.09±12.37dD 847.96±35.04eE 811.02±9.31dD1.5L/2d 914.39±44.74dD 823.74±20.07dD 854.39±77.35eE 830.03±2.04dD1.2L/2d 1382.82±35.12cC 1349.74±111.33bBC 1090.34±53.06dD 1215.40±55.55bBC0.9L/2d 1523.38±64.56bBC 1878.95±76.18aA 1499.50±56.54cC 1864.76±64.29aA0.6L/2d 1647.22±35.30bB 1458.38±55.70bB 1739.57±59.98bB 1295.55±56.49bB0.3L/2d 1850.16±101.77aA 1146.28±105.49cC 2026.99±88.60aA 1082.76±31.45cC注：同一列不同的小写字母数值间差异显著（P＜0.05），同一列不同大写字母数值间差异显著（P＜0.01）Note：There were significant differences between the different capital values of the same column (P <0.01)and there wasa significant difference between the lowercase letters in the same column (P <0.05)
本文编号：3234413
【文章来源】：安徽农业大学安徽省
【文章页数】：38 页
【学位级别】：硕士
【部分图文】：
葫芦素的生物合成途径Fig.1ThebiosyntheticroutetoCucurbitacin
注：a 为黄山老黄瓜，b 为中农 16图 2 不同水分处理下黄瓜叶片在 5d 和 15d POD 活性的变化Fig.2 The variety of cucumber leaves POD under different water treatments in fifth and fifteenth days表 1 不同水分处理下黄瓜叶片在 5d 和 15d POD 活性Table 1 POD activity of cucumber leaves under different water treatments in fifth and fifteenth days黄山老黄瓜 中农 165d 15d 5d 15d1.8L/2d 1176.67±42.49eE 1201.67±51.37cC 1265.02±32.50eE 1269.04±49.81dD1.5L/2d 1194.33±134.80eE 1251.01±88.98cC 1312.01±32.17eE 1338.67±61.15dD1.2L/2d 1760.33±48.94dD 1840.33±69.82bB 2148.33±156.01dD 2548.34±60.60bB0.9L/2d 2346.00±65.91cC 2546.02±90.61aA 2726.03±52.64cC 3189.67±99.43aA0.6L/2d 3080.33±179.35bB 1947.01±79.66bB 3343.67±100.90bB 2465.34±38.00bB0.3L/2d 3510.33±84.70aA 1909.67±99.34bB 3997.11±147.80aA 2225.68±76.56cC注：同一列不同的小写字母数值间差异显著（P＜0.05），同一列不同大写字母数值间差异显著（P＜0.01）Note：There were significant differences between the different capital values of the same column (P <0.01)and there wasa significant difference between the lowercase letters in the same column (P <0.05)
注：a 为黄山老黄瓜，b 为中农 16图 3 不同水分处理下黄瓜叶片在 5d 和 15d SOD 活性的变化Fig.3 The variety of cucumber leaves SOD under different water treatments in fifth and fifteenth days表 2 不同水分处理下黄瓜叶片在 5d 和 15d SOD 活性Table 2 SOD activity of cucumber leaves under different water treatments in fifth and fifteenth days黄山老黄瓜 中农 165d 15d 5d 15d1.8L/2d 894.66±59.14dD 736.09±12.37dD 847.96±35.04eE 811.02±9.31dD1.5L/2d 914.39±44.74dD 823.74±20.07dD 854.39±77.35eE 830.03±2.04dD1.2L/2d 1382.82±35.12cC 1349.74±111.33bBC 1090.34±53.06dD 1215.40±55.55bBC0.9L/2d 1523.38±64.56bBC 1878.95±76.18aA 1499.50±56.54cC 1864.76±64.29aA0.6L/2d 1647.22±35.30bB 1458.38±55.70bB 1739.57±59.98bB 1295.55±56.49bB0.3L/2d 1850.16±101.77aA 1146.28±105.49cC 2026.99±88.60aA 1082.76±31.45cC注：同一列不同的小写字母数值间差异显著（P＜0.05），同一列不同大写字母数值间差异显著（P＜0.01）Note：There were significant differences between the different capital values of the same column (P <0.01)and there wasa significant difference between the lowercase letters in the same column (P <0.05)
本文编号：3234413
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