外源褪黑素和油菜素内酯提高草坪草高羊茅抗热和抗旱性的机制
发布时间：2021-07-11 17:31
高羊茅（Festuca arundinacea Schreb.）是一种冷季型草坪草,对于低温的耐受性相对较强,但是高羊茅的抗热性和抗旱性在草坪草中属于较差类型。华中地区高羊茅的越夏困难,如何提高高羊茅对高温和干旱的抗性具有重要的意义。褪黑素（MT）和油菜素内酯（BR）作为两种重要的生长调节物质,都可以促进植物的生长,增强植物的抗逆性。本研究中,利用MT和2,4-油菜素内酯（EBL）处理高羊茅材料,探讨MT和EBL对高羊茅生长状态、生理指标以及基因表达的影响。研究结果如下:1.高温处理实验中,8天苗龄的高羊茅材料用20 μM MT或0.1 μM EBL预处理2天,然后在38 ℃条件下进行处理6或12小时。结果表明,在高温处理以后,MT和EBL预处理的高羊茅材料展示出较低的电导率、MDA含量、活性氧含量,包括超氧阴离子（O2-）和过氧化氢（H2O2）,但是较高的叶绿素含量、总蛋白质含量和抗氧化酶活性（包括超氧化物歧化酶、过氧化氢酶和过氧化物酶）。高温条件下MT和EBL预处理促进了高羊茅的生长,提高了植株的鲜重和植株高度。通过RNA seq分析发现,4311和8395个unigene在高温处...
【文章来源】：中国科学院大学(中国科学院武汉植物园)湖北省
【文章页数】：137 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
1. Background
1.1 Heat stress
1.1.1 Effects of heat stress on the plants
1.1.2 The roles of ROS during plant heat stress responses
1.1.3 Adaptation mechanisms of plants to hightemperature
1.1.4 Effects of exogenous compounds on plant heat stress responses
1.2 Drought stress
1.3 Melatonin
1.3.1 Functions of melatonin
1.3.2 Biosynthesis of melatonin
1.3.3 The roles of melatonin
1.4 2,4-Epibrassinolide
1.4.1 Functions of brassinosteroids
1.4.2 The roles of 2,4-epibrassinolide/brassinosteroids
1.5 Tall fescue
2. Chapter OneTranscriptomis profiling of tall fescue in response to heat stress andimproved thermotolerance by melatonin and 2,4 epibrasnolide
2.1 Introduction
2.1.1 Objectives, purposes and content research
2.2 Materials and Methods
2.2.1 Plant material and growth conditions
2.2.2 Chemicals, treatments and experimental design
2.2.3 Measurement of fresh weight and plant height of tall fescue
2.2.4 Quantification of reactive oxygen species,antioxidantenzymes and protein content
2.2.5 Measurement of malondialdehyde and electrolyte leakage
2.2.6 Measurement of chlorophyll
2.2.7 RNA sequencing analysis
2.2.8 Gene ontology (GO)term and pathway enrichment and clusteranalyses
2.2.9 Quantitative real-time-polymerasc chain reaction (qRT-PCR)
2.2.10 Statistical analysis
2.3 Result
2.3.1 Effects of MT and EBL on the plant growth under heatstress
2.3.2 Effects of MT and EBL on electrolyte leakage, chlorophyll andtotal protein content under heat tress
2.3.3 Effects of MT and EBL on ROS and cell membrane stabilityunder heat stress
2.3.4 Effects of MT and EBL priming on the activities of antioxidantenzymes under heat stress
2.3.5 General transcriptomic responses of tall fescue to heat stress
2.3.6 GO term and pathway enrichment analyses
2.3.7 Cluster analysis and functions of heat stress responsivegenes
2.3.8 Changes of hormone pathway related genes
2.3.9 Effects of MT and EBL on the expression of heat stress inducedgenes
2.4 Discussion
3 Chapter TwoMelatonin ameliorates drought stress-induced damages of tall fescue
3.1 Introduction
3.1.1 Objectives
3.2 Materials and Methods
3.2.1 Plant materials and growth conditions
3.2.2 Experimental design and drought stress treatment
3.2.3 Measurement of shoot fresh weight and shoot height of tallfescue Seedlings
3.2.4 Measurement of water loss and leaf water content
3.2.5 Quantification of reactive oxygen species and antioxidantenzymes
3.2.6 Measurement of malondialdehide and electrolyte leakage
3.2.7 Measurement of chlorophyll
3.2.8 Statistical analysis
3.3 Result
3.3.1 Effects of exogenous melatonin an growth conditions of tallfescue seedling
3.3.2 Effects of exogenous melatonin on electrolyte leakage and totalchlorophyll content
3.3.3 Effects of exogenous melatonin on shoot water loss and shootwater content
3.3.4 Effects of exogenous melatonin on ROS and cell membranestability
3.3.5 Effects of exogenous melatonin on the antioxidant enzymeactivities
3.4 Discussion
4. Conclusion
5. References
Appendices
Student profile
Acknowledgement
【参考文献】：
期刊论文
[1]Interactive effects of drought stress and chitosan application on physiological characteristics and essential oil yield of Thymus daenensis Celak[J]. Zohreh Emami Bistgani,Seyed Ataollah Siadat,Abdolmehdi Bakhshandeh,Abdollah Ghasemi Pirbalouti,Masoud Hashemi. The Crop Journal. 2017(05)
[2]外源褪黑素对高温胁迫条件下黄瓜幼苗氮代谢的影响[J]. 赵娜,孙艳,王德玉,郑俊鶱. 植物生理学报. 2012(06)
本文编号：3278537
【文章来源】：中国科学院大学(中国科学院武汉植物园)湖北省
【文章页数】：137 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
1. Background
1.1 Heat stress
1.1.1 Effects of heat stress on the plants
1.1.2 The roles of ROS during plant heat stress responses
1.1.3 Adaptation mechanisms of plants to hightemperature
1.1.4 Effects of exogenous compounds on plant heat stress responses
1.2 Drought stress
1.3 Melatonin
1.3.1 Functions of melatonin
1.3.2 Biosynthesis of melatonin
1.3.3 The roles of melatonin
1.4 2,4-Epibrassinolide
1.4.1 Functions of brassinosteroids
1.4.2 The roles of 2,4-epibrassinolide/brassinosteroids
1.5 Tall fescue
2. Chapter OneTranscriptomis profiling of tall fescue in response to heat stress andimproved thermotolerance by melatonin and 2,4 epibrasnolide
2.1 Introduction
2.1.1 Objectives, purposes and content research
2.2 Materials and Methods
2.2.1 Plant material and growth conditions
2.2.2 Chemicals, treatments and experimental design
2.2.3 Measurement of fresh weight and plant height of tall fescue
2.2.4 Quantification of reactive oxygen species,antioxidantenzymes and protein content
2.2.5 Measurement of malondialdehyde and electrolyte leakage
2.2.6 Measurement of chlorophyll
2.2.7 RNA sequencing analysis
2.2.8 Gene ontology (GO)term and pathway enrichment and clusteranalyses
2.2.9 Quantitative real-time-polymerasc chain reaction (qRT-PCR)
2.2.10 Statistical analysis
2.3 Result
2.3.1 Effects of MT and EBL on the plant growth under heatstress
2.3.2 Effects of MT and EBL on electrolyte leakage, chlorophyll andtotal protein content under heat tress
2.3.3 Effects of MT and EBL on ROS and cell membrane stabilityunder heat stress
2.3.4 Effects of MT and EBL priming on the activities of antioxidantenzymes under heat stress
2.3.5 General transcriptomic responses of tall fescue to heat stress
2.3.6 GO term and pathway enrichment analyses
2.3.7 Cluster analysis and functions of heat stress responsivegenes
2.3.8 Changes of hormone pathway related genes
2.3.9 Effects of MT and EBL on the expression of heat stress inducedgenes
2.4 Discussion
3 Chapter TwoMelatonin ameliorates drought stress-induced damages of tall fescue
3.1 Introduction
3.1.1 Objectives
3.2 Materials and Methods
3.2.1 Plant materials and growth conditions
3.2.2 Experimental design and drought stress treatment
3.2.3 Measurement of shoot fresh weight and shoot height of tallfescue Seedlings
3.2.4 Measurement of water loss and leaf water content
3.2.5 Quantification of reactive oxygen species and antioxidantenzymes
3.2.6 Measurement of malondialdehide and electrolyte leakage
3.2.7 Measurement of chlorophyll
3.2.8 Statistical analysis
3.3 Result
3.3.1 Effects of exogenous melatonin an growth conditions of tallfescue seedling
3.3.2 Effects of exogenous melatonin on electrolyte leakage and totalchlorophyll content
3.3.3 Effects of exogenous melatonin on shoot water loss and shootwater content
3.3.4 Effects of exogenous melatonin on ROS and cell membranestability
3.3.5 Effects of exogenous melatonin on the antioxidant enzymeactivities
3.4 Discussion
4. Conclusion
5. References
Appendices
Student profile
Acknowledgement
【参考文献】：
期刊论文
[1]Interactive effects of drought stress and chitosan application on physiological characteristics and essential oil yield of Thymus daenensis Celak[J]. Zohreh Emami Bistgani,Seyed Ataollah Siadat,Abdolmehdi Bakhshandeh,Abdollah Ghasemi Pirbalouti,Masoud Hashemi. The Crop Journal. 2017(05)
[2]外源褪黑素对高温胁迫条件下黄瓜幼苗氮代谢的影响[J]. 赵娜,孙艳,王德玉,郑俊鶱. 植物生理学报. 2012(06)
本文编号：3278537
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