西瓜种子重要性状QTL分析

发布时间：2021-07-22 13:55

　　随着家庭和工业对健康植物油及其产品需求的不断增长,可通过分子育种技术从源头上寻找替代品以满足这种需求。近期,CAPS（Cleaved Amplified Polymorphism Sequence）标记已成为分子育种试验重要的分子标记技术。本试验选用两个西瓜品系材料,分别命名为P₁=W1-1（低油份含量）,P₂=PI-186490（高油份含量）,利用F₁和F_2:3代果实测量种子含油率,同时测量其他的种子表型性状（种子长度、种子厚度、种子宽度、种皮厚度、种子百粒重）。PI-186490中种子含油率（SOP）的平均值（平均值为22.25%）显着高于W1-1（平均值为14.75%）。F_2:3代的种子含油率范围为9%至32%。P₁和P₂中其他种子性状的平均值如下:种子长度（8.81 mm、14.71 mm）,种子宽度（5.96mm、10.53mm）,种子厚度（2.06 mm、2.65 mm）,种皮厚度（0.33mm、0.15 mm）,百粒重（... 

【文章来源】：东北农业大学黑龙江省 211工程院校

【文章页数】：54 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
1 Introduction
    1.1 Watermelon cultivation areas and classification
    1.2 Seed nutritional significance in watermelon
    1.3 Vegetable oils and nutrition
    1.4 Seed oil percentage and molecular markers
    1.5 QTL Analysis of Fruits and Seeds Characterization in Vegetables Crops
    1.6 Study objectives
    1.7 Technology route for research
    1.8 Research plan calendar
2 Materials and methods
    2.1 Plant materials for experiment
    2.2 Experimental design in greenhouse
    2.3 Recording of phenotypic traits
        2.3.1 Seed length, seed width and seed thickness
        2.3.2 Thickness of seed coat
        2.3.3 100 seed weight
        2.3.4 Extraction of seed oils
    2.4 Physicochemical properties experiments
        2.4.1 Fatty acid methyl ester preparation
        2.4.2 Chromatography analysis
        2.4.3 Chemical properties
    2.5 Molecular analysis experiment
        2.5.1 DNA extraction and polymerase chain reaction (PCR)
        2.5.2 Instruments
        2.5.3 Reagent for molecular analysis experiments
    2.6 Statistical analysis
3 Results and analysis
    3.1 Screening of CAPS markers for polymorphism analysis
    3.2 Fatty acid composition analysis
    3.3 Physicochemical properties analysis
    3.4 Linkage map construction analysis
    3.5 Phenotypic traits analysis
        3.5.1 Seed length analysis
        3.5.2 Seed width analysis
        3.5.3 Seed thickness analysis
        3.5.4 Thickness of seed coat analysis
        3.5.5 100 seed weight analysis
        3.5.6 Seed oil percentage analysis
    3.6 Detection of QTLs and analysis
        3.6.1 Seed length QTLs analysis
        3.6.2 Seed width QTLs analysis
        3.6.3 Seed Thickness QTL analysis
        3.6.4 Thickness of seed coat QTL analysis
        3.6.5 100 seed weight QTL analysis
        3.6.6 Seed oil percentage QTL analysis
4 Discussion
    4.1 Fatty acid composition
    4.2 Oil percentage and fatty acids correlation
    4.3 Physicochemical properties
        4.3.1 Acid value and free fatty acid
        4.3.2 Oxidizability value (Cox)
        4.3.3 Saponification value
        4.3.4 Specific gravity
    4.4 Linkage map construction with CAPS markers
    4.5 Detection of watermelon seed traits QTLs
        4.5.1 Seed length QTL
        4.5.2 Seed width QTL
        4.5.3 Seed thickness QTL
        4.5.4 Thickness of seed coat QTL
        4.5.5 100 seed weight QTL
        4.5.6 Seed oil percentage QTL
5 Conclusion
    5.1 Fatty acids
    5.2 Physicochemical properties
    5.3 Linkage map construction and QTL ana lysis
Acknowledgement
References
Appendix
Papers published in the period of Ph.M. education


【参考文献】：
期刊论文
[1]基于CAPS标记的西瓜果实与种子相关性状QTL分析[J]. 迟莹莹,高鹏,朱子成,栾非时,李桂英,于鹏.  中国农业科学. 2017(07)



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