生物调节剂对无土栽培黄瓜生长的影响

发布时间：2021-10-09 06:50

　　本研究旨在筛选各种植物生物调节剂作为添加剂,检测其在无土栽培条件下通过根系施用对黄瓜生长的影响;然后探究最佳三种添加剂腐胺（Put）、细胞分裂素（MT）、油菜素内酯（EBR）复混配比对黄瓜生长生理形态及根系活力的影响;最后,研究（5 mM Put+0.02 ppm MT+0.001 ppm EBR）对盐胁迫下黄瓜抗氧化酶的影响,并研究其对盐胁迫下黄瓜植株产量,果实品质和碳氮代谢的影响。1-试验一、不同生长调节剂对黄瓜植株生长的影响本试验共设15个处理:T1（水）、T2（10mM腐胺）、T3（250 ppm海藻素）、T4（0.02ppm细胞分裂素）、T5（100ppm萘乙酸）、T6（400 ppm聚天冬氨酸）、T7（50ppm硝基苯酚钠）、T8（100ppm L-色氨酸）、T9（1%腐殖酸）、T10（10⁷CFU/ml枯草芽孢杆菌）、T11（10⁶CFU/ml木霉菌）、T12（50 ppm L-丙氨酸）、T13（150 ppm水杨酸）、T14（1mM纳米硅）和T15（0.001 ppm油菜素内酯）,将上述生物调节剂分别加入基质中混匀,装盆,定植... 

【文章来源】：中国农业科学院北京市

【文章页数】：104 页

【学位级别】：博士

【文章目录】：
摘要
Abstract
LIST OF ABBREVIATIONS
CHAPTER 1 INTRODUCTION
    1.1 Cumcumber(cucumis sativus L.)
    1.2 Soilless plant cultivation system
    1.3 Plant bio-stimulants
    1.4 Effect of salinity stress
    1.5 Nitrogen assimilation and enzymes activities
        1.5.1 Assimilation of inorganic nitrogen into glutamine and glutamate
        1.5.2 Primary nitrogen assimilation
        1.5.3 Glutamine synthase
        1.5.4 Glutamate synthase
        1.5.5 Glutamate dehydrogenase
    1.6 Nitrogen carbon metabolism relationship
    1.7 Significance of the Study
    1.8 Objectives of the Study
CHAPTER 2:Effect of Various Plant Bio-stimulants on Cucumber Growth
    2.1 Summary
    2.2 Introduction
    2.3 Materials and methods
        2.3.1 Experiment treatments
        2.3.2 Morphological parameters
            2.3.2.1 Plant growth
            2.3.2.2 Seedling vigor index(SVI)
        2.3.3 Net Photosynthetic rate
        2.3.4 Statistical Analysis
    2.4 Results
        2.4.1 Morphological parameters
            2.4.1.1 Plant height
            2.4.1.2 Leaf numbers
            2.4.1.3 Stem diameter
            2.4.1.4 Leaf area
            2.4.1.5 Cucumber seedlings vigor
        2.4.2 Shoot and root fresh weight
        2.4.3 Photosynthetic rate(Pn)after30 days
    2.5 Discussion
    2.6 Conclusion
CHAPTER 3:Effect of Plant Growth Regulators Combinations(PGRsc),on Cucumber Growth under Soilless Culture
    3.1 Summary
    3.2 Introduction
    3.3 Materials and methods
        3.3.1 Plant materials and growth condition
        3.3.2 The experiment design and treatments
        3.3.4 Morphological parameters
            3.3.4.1 Plant growth
            3.3.4.2 Seedlings vigor
        3.3.5 Physiological parameters
            3.3.5.1 Chlorophyll content
            3.3.5.2 Net photosynthetic rate(pn)
        3.3.6 Root activity(TTC)
        3.3.7 Statistical Analysis
    3.4 Results
        3.4.1 Morphological parameters
        3.4.2 Seedlings vigor index(SVI)
        3.4.3 Physiological parameters
            3.4.3.1 Total chlorophyll content
            3.4.3.2 Net photosynthetic rate(pn)
        3.4.4 Root activity
    3.5 Discussion
    3.6 Conclusion
CHAPTER 4:Effect of Plant Growth regulators Combination(PGRsc),on Antioxidant enzymes activities in Cucumber plant under NaCl stress
    4.1 Summary
    4.2 Introduction
    4.3 Materials and methods
        4.3.1 The experiment design and treatments
        4.3.3 Morphological parameters
        4.3.4 Root activity
        4.3.5 Protein content
        4.3.6 Determination of malondialdehyde(MDA)contents,proline and antioxidant enzymes activities
        4.3.7 Electrolyte leakage
        4.3.8 Data recorded and statistical analysis
    4.4 Results
        4.4.1 Morphological parameters
        4.4.2 Root activity
        4.4.3 Soluble protein content in leaves
        4.4.4 Proline content in leaves and roots
        4.4.5 Malondialdehyde(MDA)content in leaf
        4.4.6 Antioxidant enzymes activities
        4.4.7 Electrolyte leakage
    4.5 Discussion
    4.6 Conclusion
CHAPTER 5:Effect of Plant Growth Regulators Combination(PGRsc)on Nitrogen/ Carbon metabolism and Fruit Quality of Cucumber under Salt Stress
    5.1 Summary
    5.2 Introduction
    5.3 Materials and methods
        5.3.1 The experiment design and treatments
        5.3.2 Morphological parameters
        5.3.3 Determination of fruit yield and quality parameters
            5.3.3.1 Early fruit yield
            5.3.3.2 Vitamin C,soluble sugar and Protein contents in cucumber fruits
        5.3.4 Total nitrogen
        5.3.5 Nitrogen and carbohydrate enzymes
        5.3.6 Data recorded and statistical analysis
    5.4 Results
        5.4.1 Plant biomass
        5.4.2 Early fruit yield
        5.4.3 Vitamin C(AsA)
        5.4.4 Protein content
        5.4.6 Total soluble sugar
        5.4.7 Total nitrogen
        5.4.8 Nitrogen enzymes activities in leaves and roots
        5.4.9 Carbohydrate enzymes in leaves and roots
    5.5 Discussion
    5.6 Conclusion`
Chapter 6:Conclusion
REFERENCES
ACKNOWLEDGEMENTS
CURRICULUM VITAE



本文编号：3425856