应用昆虫分子系统学对法医嗜尸性蝇类种属鉴定的研究
发布时间：2018-08-01 08:20
【摘要】：目的 本课题旨在探索可用于法医嗜尸性蝇类种属鉴定的昆虫分子系统学方法。方法 选择线粒体DNA(mtDNA)Cytb和ND1基因，PCR扩增，聚丙烯酰胺凝胶电泳银染显色分型，用DNA遗传分析仪对分型产物测序；利用系统进化学软件和统计学方法对序列进行分析，建立对法医嗜尸性蝇类进行种属鉴定的方法。结果 对中国3个地区6种嗜尸性蝇类的mtDNA Cytb和ND1基因进行了扩增、电泳检测和序列分析；利用统计学的方法，通过系统进化学的分析，建立了这些嗜尸性蝇类的系统进化关系；结合系统进化学的分析结果，初步建立了法医嗜尸性蝇类种属鉴定的方法。结论 基于mtDNA Cytb和ND1基因，初步建立了法医嗜尸性蝇类种属鉴定的昆虫分子系统学方法。
[Abstract]:Objective to explore a molecular phylogenetic method for identification of necrophilic flies in forensic medicine. Methods Mitochondrial DNA (mtDNA) Cytb and ND1 gene were amplified by PCR, polyacrylamide gel electrophoresis (page) silver staining was used to type the genotypes, DNA genetic analyzer was used to sequence the typing products, and the sequence was analyzed by systematic chemical software and statistical method. A method for species identification of necrophilic flies in forensic medicine was established. Results the mtDNA Cytb and ND1 genes of 6 species of necrophilic flies in three regions of China were amplified, electrophoretic and sequenced. The phylogenetic relationship of these necrophagous flies was established, and the method of identifying the species of coriophilic flies by forensic medicine was preliminarily established in combination with the results of phylochemical analysis. Conclusion based on mtDNA Cytb and ND1 genes, a molecular phylogenetic method for identification of species of necrophilic flies was established.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919.4
本文编号：2156943
[Abstract]:Objective to explore a molecular phylogenetic method for identification of necrophilic flies in forensic medicine. Methods Mitochondrial DNA (mtDNA) Cytb and ND1 gene were amplified by PCR, polyacrylamide gel electrophoresis (page) silver staining was used to type the genotypes, DNA genetic analyzer was used to sequence the typing products, and the sequence was analyzed by systematic chemical software and statistical method. A method for species identification of necrophilic flies in forensic medicine was established. Results the mtDNA Cytb and ND1 genes of 6 species of necrophilic flies in three regions of China were amplified, electrophoretic and sequenced. The phylogenetic relationship of these necrophagous flies was established, and the method of identifying the species of coriophilic flies by forensic medicine was preliminarily established in combination with the results of phylochemical analysis. Conclusion based on mtDNA Cytb and ND1 genes, a molecular phylogenetic method for identification of species of necrophilic flies was established.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919.4
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