薄层色谱法和高效液相色谱法综合考察签字笔油墨

发布时间：2018-08-25 08:30

【摘要】： 随着计算机和网络的发展，电子通信，电子署名和整体电子经济业务变得越来越流行，但是笔墨书写的文件依然是人们在商业交往中广泛使用和信任的法律文件。因此，可疑文件的检测依然是法庭科学中的重要课题。本文主要针对国内外不同生产厂家的90种黑色签字笔和94种蓝色签字笔进行色谱法分类的研究，主要包括薄层色谱法(TTC)和高效液相色谱法(HPLC)。首先，本文概述了油墨的主要成分，包括染料、溶剂、树脂、抗氧化剂，耐热剂、防水剂以及耐光剂等。并对薄层色谱法、高效液相色谱法在该领域的研究进行了总结。其次，通过薄层色谱法、高效液相色谱法对样品笔进行了系统的分析。其中，薄层色谱法由于操作方便、廉价、快速等优点得到广泛应用。根据油墨中不同的化学组分在薄层板上产生的斑点的个数、颜色以及比移值的不同对签字笔进行分类。根据本实验的薄层色谱条件，将黑色签字笔分为9类，蓝色签字笔分为11类。薄层色谱法分辨能力有其局限性，无法区分组分的微小的差异，而高效液相色谱法则具有更高的灵敏度和分辨率，，因此，研究了高效液相色谱法对这些签字笔进行进一步分类的可能性。最后在实验中，找到了分别适合黑色签字笔和蓝色签字笔分析的色谱条件。使用Purospher STAR C18色谱柱，在黑色签字笔和蓝色签字笔的流动相中分别加入了四丁基氢氧化铵离子对试剂(0.004g／mL，pH6.5)和碳酸氢铵缓冲盐(10mmol／L，pH8.0)，二极管阵列检测器对400-700nm进行扫描。最后，在薄层色谱分析结果的基础上，进一步分析黑色签字笔和蓝色签字笔。
[Abstract]:With the development of computer and network, electronic communication, electronic signature and the whole electronic economic business become more and more popular, but the documents written by pen and ink are still the legal documents widely used and trusted by people in business exchanges. Therefore, the detection of suspicious documents is still an important subject in forensic science. This paper focuses on the classification of 90 black and 94 blue signature pens from different manufacturers at home and abroad, including thin layer chromatography (TLC) (TTC) and high performance liquid chromatography (HPLC). Firstly, this paper summarizes the main components of ink, including dyes, solvents, resins, antioxidants, heat resistant agents, water repellents and light resistant agents. The research of thin-layer chromatography and high-performance liquid chromatography in this field were summarized. Secondly, the sample pen was systematically analyzed by TLC and HPLC. Among them, TLC has been widely used because of its advantages of convenient operation, cheap and fast operation. The pen is classified according to the number of spots produced on the thin plate by different chemical components in the ink, the color and the value of specific shift. According to the TLC conditions of the experiment, the black pen is divided into 9 types and the blue pen is divided into 11 categories. The resolution ability of TLC has its limitations, and it can not distinguish the tiny difference of components, while HPLC has higher sensitivity and resolution, so, The possibility of further classification of these pens by high performance liquid chromatography (HPLC) was studied. Finally, the chromatographic conditions suitable for the analysis of black pen and blue pen were found. Using Purospher STAR C18 column, tetraDing Ji ammonium hydroxide ion pair reagent (0.004 g / mL ~ (-1) pH 6.5) and ammonium bicarbonate buffer salt (10 mmol / L ~ (-1) pH 8.0) were added to the mobile phase of black and blue pen respectively. The 400-700nm was scanned by diode array detector. Finally, based on the results of TLC analysis, black and blue signature pens were further analyzed.
【学位授予单位】：首都师范大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：D918.2

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