大鼠骨骼肌肌动蛋白在死亡后的形态学变化

发布时间：2018-08-27 18:18

【摘要】：目的探讨大鼠骨骼肌肌动蛋白纤维的形态学变化与死亡时间的相关性。方法分别采用激光扫描共聚焦显微镜和透射电镜对大鼠死后不同时间骨骼肌(右后肢的内收大肌)肌动蛋白纤维的形态学变化进行观察。结果大鼠死后,透射电镜观察可见由肌动蛋白构成的细肌丝逐渐崩解、紊乱,直至肌小节和细肌丝结构消失;激光扫描共聚焦显微镜观察可见,自死后24h起,骨骼肌纤维横纹可有小片状或弥漫状抗肌动蛋白抗体缺染灶出现,并且抗肌动蛋白抗体染色面积随着死亡时间的延长而逐渐减少,其变化趋势与死亡时间相关(Y=0.934-0.005X,R2=0.95,P0.05);大鼠死后168h内,肌动蛋白阳性产物的积分光密度值随死亡时间的延长而逐渐降低,组间差异均具有统计学意义(P0.05),至死后168h,几乎无抗肌动蛋白抗体染色。结论大鼠骨骼肌肌动蛋白纤维的形态学改变与死亡时间具有相关性。
[Abstract]:Objective to investigate the relationship between the morphological changes of actin fibers and the time of death in rat skeletal muscle. Methods the morphologic changes of actin fibers in skeletal muscle (adductor greater adductor muscle of right hind limb) were observed by laser scanning confocal microscope and transmission electron microscope respectively. Results after death, the fine myofilaments composed of actin gradually disintegrated and disorganized, and the structure of the sarcomere and the fine myofilament disappeared, and the laser scanning confocal microscopy showed that the fine myofilament was disintegrated from 24 hours after death. In skeletal muscle fibers, small or diffuse anti-actin antibodies were absent, and the staining area of anti-actin antibodies gradually decreased with the prolongation of death time, and the change trend was related to the death time (P 0.05), and within 168h after death, the change trend of anti-actin antibody was related to the death time (P 0.05), and the changes of the staining area of anti-actin antibody were correlated with the death time (P 0.05), and the changes of the staining area of anti-actin antibody were correlated with the death time (P 0.05). The integral optical density of actin positive products decreased gradually with the prolongation of death time. The difference between groups was statistically significant (P0.05). There was almost no anti-actin antibody staining at 168h after death. Conclusion the morphological changes of actin fibers in rat skeletal muscle are correlated with the time of death.
【作者单位】：山西中医学院病理学教研室;山西医科大学法医学院;
【分类号】：D919

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