基于mRNA稳定性推断死亡时间的研究
发布时间：2018-09-18 14:47
【摘要】：目的探讨大鼠死后不同时间肾、脾3-磷酸甘油醛脱氢酶(GAPDH)mRNA的表达对死亡时间(PMI)推断的应用价值。方法28只大鼠断颈处死后置于20℃温控系统内,分别于死后0,8,16,24,32,40,48h共7个时相点(n=4)解剖,利用荧光RT-PCR技术检测大鼠肾、脾GAPDHmRNA相对表达量的变化。结果在大鼠死后40h肾、32h脾内可检测出GAPDHmRNA,且其表达产物均呈规律性下降趋势(荧光强度逐渐变暗并最终消失)。结论检测大鼠死后不同时间肾、脾GAPDHmRNA的表达对PMI推断具有一定的应用价值。
[Abstract]:Objective to investigate the value of the expression of 3-phosphoglyceraldehyde dehydrogenase (GAPDH) mRNA) in the kidney and spleen of rats at different time after death in predicting the death time (PMI). Methods 28 rats were killed by cervical amputation and placed in the temperature control system at 20 鈩，
本文编号：2248248
[Abstract]:Objective to investigate the value of the expression of 3-phosphoglyceraldehyde dehydrogenase (GAPDH) mRNA) in the kidney and spleen of rats at different time after death in predicting the death time (PMI). Methods 28 rats were killed by cervical amputation and placed in the temperature control system at 20 鈩，
本文编号：2248248
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