小鼠皮肤切创愈合过程中caspase-9和caspase-3表达及其时间规律性变化的研究

发布时间：2018-11-08 13:55

【摘要】：前言皮肤损伤愈合大致分为三个阶段,即炎症期、纤维增生期和组织重建期。多核粒细胞、单核细胞和成纤维细胞参与皮肤损伤愈合的全过程,清除损伤、坏死的皮肤组织和入侵的病原体并重建损伤组织,对于损伤愈合具有重要作用。在炎症期和纤维增生期损伤区内多核粒细胞、单核细胞和成纤维细胞的数量急剧增加;在组织重建期,这三种细胞的数量则显著减少,直至伤口完全愈合。研究表明,这种细胞的消亡是通过凋亡(apoptosis)机制来完成的。作为细胞凋亡调节因子家族,半胱氨酸天冬氨酸特异性蛋白酶(cysteine asparatic acid-specific proteases, caspase)家族在凋亡的执行过程中占据着中心地位,直接参与凋亡启动、信号传递及凋亡效应。在caspase家族中,caspase-9(又称Apaf-3;ICE-LAP6;MCH6)按其功能和结构分类属于启动子caspase(initiator caspase),能在多种内外源性刺激因子的作用下表达,通过线粒体通路激活其效应子caspase-3,从而诱导细胞凋亡。在小鼠皮肤切创愈合过程中,损伤及损伤周边区内多核粒细胞、单核细胞和成纤维细胞是否表达caspase-9目前国内外尚未见研究报道。本实验应用免疫组织化学染色技术对小鼠皮肤切创愈合过程中多核粒细胞、单核细胞和成纤维细胞中caspase-9和caspase-3的表达情况进行了研究,并采用Western blot技术检测caspase-9在切创皮肤中的表达情况,以推断caspase-9在皮肤切创愈合过程中的生物学功能,并对其表达时间变化规律在推断损伤时间中的可应用性进行了探讨。
[Abstract]:The healing of skin injury is divided into three stages: inflammation stage, fibrous hyperplasia stage and tissue reconstruction stage. Polymorphonuclear granulocytes monocytes and fibroblasts are involved in the whole process of skin injury healing by removing the damaged necrotic skin tissue and invading pathogens and reconstructing the injured tissue which plays an important role in wound healing. The number of monocytes and fibroblasts increased sharply in the injury zone of inflammatory and fibroproliferative phase, but decreased significantly during tissue reconstruction until the wound healed completely. Studies have shown that the death of this cell is accomplished by apoptotic (apoptosis) mechanism. As a family of apoptosis regulators, cysteine aspartate specific protease (cysteine asparatic acid-specific proteases, caspase) family plays a central role in the process of apoptosis, directly involved in apoptosis initiation, signal transduction and apoptosis effect. In the caspase family, caspase-9 (also known as Apaf-3;ICE-LAP6;) MCH6) belongs to the promoter caspase (initiator caspase), according to its functional and structural classification, which can be expressed under the action of multiple endogenous stimulators, and its effector caspase-3, can be activated by mitochondrial pathway to induce cell apoptosis. During the healing process of skin incision in mice, the expression of caspase-9 in PMN, monocytes and fibroblasts in the injured and injured peripheral areas has not been reported at home and abroad. The expression of caspase-9 and caspase-3 in multinucleated granulocytes, monocytes and fibroblasts during skin wound healing in mice was studied by immunohistochemical staining. Western blot technique was used to detect the expression of caspase-9 in the skin, to infer the biological function of caspase-9 in the healing process of skin incision, and to explore the applicability of caspase-9 expression time change in the estimation of injury time.
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919

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