大鼠肌肉钝挫伤vWF及VEGF表达的实验研究

发布时间：2018-11-26 12:14

【摘要】：研究背景及目的:在法医学实践中,历来对损伤时间的推断建立在创伤修复反应的病理生理学基础之上,即以不同经历时间的创口在修复过程中所表现的生活反应(vital reaction)特点——炎症反应、肉芽组织及上皮增生和疤痕修复所产生的各种形态、组化及生化变化特征作为推断损伤时间和性质的依据。机体损伤大多导致血管内皮的损伤,其直接后果是凝血过程的启动及其后的组织充血水肿,炎细胞浸润,组织修复再生等,其间von Willebrand Factor(vWF)对损伤血管内皮部位血小板的粘附聚集起到了重要作用;血管内皮生长因子(Vascular endothelial growth facto, VEGF)是作用最强,特异性最高的血管生成调控因子,在损伤的早期起到调节血管内皮细胞通透性,趋化炎细胞的作用,在修复期起改变细胞外基质,使其更易于血管的生长。在创伤的修复过程中,细胞—生长因子—细胞外基质(EcM, extracellular matrix)三者相互作用、相互影响的复杂动态过程,生长因子参与了创伤愈合的整个过程并在其中发挥着举足轻重的作用。创伤修复过程中,内源性生长因子起着重要的调控作用,因而检查创伤后机体内的凝血因子及生长因子的改变,使更加精确地推断损伤时间成为可能。方法:健康Sprague-Dawley大鼠(S-D大鼠)65只,随机分为生前伤组、死后伤组和正常对照组,每组5只:生前伤组按处死时间不同分为15min、30min、1h、3h、6h、12h、1d、3d、5d、7d共十个亚组;
[Abstract]:Background and objective: in forensic practice, the estimation of injury time has always been based on the pathophysiological basis of wound repair response. That is, the (vital reaction) characteristics of life response in the repair process of wounds over different periods of time-inflammatory reactions, granulation tissue and epithelial hyperplasia, and various forms of scar repair. Histochemical and biochemical changes were used as the basis for estimating the time and nature of injury. The direct consequence of the injury is the initiation of coagulation process and the subsequent hyperemia and edema, inflammatory cell infiltration, tissue repair and regeneration, etc. Von Willebrand Factor (vWF) plays an important role in platelet adhesion and aggregation in injured vascular endothelium. Vascular endothelial growth factor (Vascular endothelial growth facto, VEGF) is the strongest and most specific angiogenesis regulator, which regulates the permeability and chemotaxis of vascular endothelial cells in the early stage of injury. The extracellular matrix is altered during the repair phase to make it easier for blood vessels to grow. In the process of wound repair, the complex dynamic process of interaction of cell-growth factor-extracellular matrix (EcM, extracellular matrix), Growth factor is involved in the whole process of wound healing and plays an important role in it. Endogenous growth factor plays an important role in the process of wound repair, so it is possible to detect the changes of coagulation factor and growth factor in the body after trauma, so it is possible to infer the injury time more accurately. Methods: 65 healthy Sprague-Dawley rats (S-D rats) were randomly divided into antemortem injury group, postmortem injury group and normal control group with 5 rats in each group. There were ten subgroups in 7 days.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2004
【分类号】：D919

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