死后小鼠肾组织细胞核DNA变化规律的研究

发布时间：2019-01-18 12:52

【摘要】：目的:研究DNA降解变化与死亡时间的关系,为法医学推断死亡时间提供一种比较准确可靠的新方法。方法:应用单细胞凝胶电泳(SCGE)技术结合荧光显微镜和专业的计算机图像分析技术,测定111只小鼠在死后72h内不同时间点小鼠肾组织细胞核头半径、尾长度、头DNA含量比例、尾DNA含量比例、尾矩、Olive矩、头面积、尾面积8项参数的变化值。结果:在个体死亡72h内,测定的8项参数指标中尾DNA含量比例、彗星尾长、尾矩、Olive矩、尾面积都呈增加趋势,头半径、头DNA含量比例、头面积均呈下降趋势。上述参数均与死亡时间具有高度的相关性。并将每个参数的测量值进行了多项式运算,获得了更能体现DNA降解趋势的二项式回归方程和多元回归方程,均具有高度的统计学意义(P均0.01)。结论:应用本研究提供的72h内肾组织组织DNA变化与死亡时间之间呈线性关系的各组回归方程,为法医学推断死后经过时间提供了一种新的、客观的、精确的方法和参考依据。
[Abstract]:Objective: to study the relationship between DNA degradation and time of death, and to provide a new accurate and reliable method for estimating the time of death in forensic medicine. Methods: single cell gel electrophoresis (SCGE) technique combined with fluorescence microscope and computer image analysis technique were used to determine the head radius, tail length and DNA content of renal tissue of 111 mice at different time points within 72 hours after death. Ratio of tail DNA content, tail moment, Olive moment, head area and tail area. Results: within 72 hours of death, the ratio of tail DNA content, comet tail length, tail moment, Olive moment and tail area showed an increasing trend, and the head radius, head DNA content ratio and head area showed a decreasing trend. The above parameters were highly correlated with the time of death. The polynomial calculation of the measured values of each parameter was carried out and the binomial regression equation and the multivariate regression equation which could reflect the trend of DNA degradation were obtained which had high statistical significance (P0.01). Conclusion: the regression equation between the changes of DNA in renal tissue and the time of death in 72 h was used to provide a new, objective, accurate method and reference for forensic science to deduce postmortem time.
【作者单位】：中国医科大学基础医学院化学教研室中国刑警学院法医系中国刑警学院法医系中国刑警学院法医系
【基金】：公安部科研基金资助项目(1997-2C)
【分类号】：D919

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