大鼠皮肤切创愈合过程中iNOS和eNOS表达的时序性变化
发布时间：2019-01-23 07:57
【摘要】：目的:观察大鼠皮肤切创愈合过程中诱导型一氧化氮合酶(inducible nitric oxide synthases, iNOS)和内皮型一氧化氮合酶(endothelial nitric oxide synthases, eNOS)蛋白表达的变化,以探讨其在法医实践中用于推断损伤时间的可能性。 方法:建立大鼠皮肤切创伤模型,应用免疫组织化学技术(SP 法)和图像分析方法,研究不同损伤时间(生前伤1h-14d) 大鼠皮肤创缘组织中iNOS 和eNOS 蛋白表达的变化及死后不同时间对iNOS 和eNOS 蛋白的影响。 结果:(1)生前伤不同时间iNOS 蛋白表达的变化:各时间点创口对侧组织未见iNOS 表达。大鼠皮肤切创伤后角朊细胞、汗腺、毛囊、和骨骼肌细胞及创伤后肉芽组织的炎细胞、成纤维细胞、血管内皮细胞均不同程度的表达iNOS 蛋白,免疫组化染色iNOS 蛋白呈棕黄色颗粒样分布于上述细胞胞浆。①肉芽组织: 创伤后6h,创缘附近浸润的巨噬细胞、中性粒细胞开始表达iNOS 蛋白,呈弱阳性染色;12h,阳性染色的巨噬细胞和中性粒细胞增多;创伤后1d,iNOS 蛋白表达量达到高峰,并以较高水平维持至伤后5d, 可见到强阳性颗粒分布于炎细胞、成纤维细胞、血管内皮细胞胞浆;7d-14d,仍可见到成纤维细胞、纤维细胞、新生毛细血管内皮细胞表达iNOS 蛋白,但阳性细胞数目逐渐减少,且染色程度逐渐减弱。②表皮:创伤后1h, 创缘角朊细胞表达微量
[Abstract]:Objective: to observe the expression of inducible nitric oxide synthase (inducible nitric oxide synthases, iNOS) and endothelial nitric oxide synthase (endothelial nitric oxide synthases, eNOS) protein in the healing process of rat skin incision. To explore the possibility of its use in forensic practice to infer the time of injury. Methods: the rat skin injury model was established. Immunohistochemical technique (SP) and image analysis were used. To study the changes of iNOS and eNOS protein expression in the injured skin tissue of rats with different injury time (1h-14d) and the effect of different postmortem time on iNOS and eNOS protein. Results: (1) the changes of iNOS protein expression at different time points: no iNOS expression was found in the contralateral tissue of wound at different time points. INOS protein was expressed in posttraumatic keratinocytes, sweat glands, hair follicles, inflammatory cells of skeletal muscle cells and posttraumatic granulation tissue, fibroblasts and vascular endothelial cells in rats. Immunohistochemical staining showed that iNOS protein distributed in the cytoplasm of the above cells as brown granules. 1 granulation tissue: at 6 hours after trauma, macrophages infiltrated near the wound edge, neutrophils began to express iNOS protein and showed weak positive staining. At 12 h, the number of positive staining macrophages and neutrophils increased. The expression of iNOS reached its peak on the 1st day after trauma, and maintained at a high level until 5 days after injury. The strong positive granules were found in the cytoplasm of inflammatory cells, fibroblasts and vascular endothelial cells. At 7 d to 14 d, fibroblasts could still be seen. INOS protein was expressed in neocapillary endothelial cells, but the number of positive cells gradually decreased and the staining degree gradually weakened. 2 epidermis: 1 hour after trauma, microamounts of keratinocytes expressed in wound edge keratinocytes
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：D919
本文编号：2413612
[Abstract]:Objective: to observe the expression of inducible nitric oxide synthase (inducible nitric oxide synthases, iNOS) and endothelial nitric oxide synthase (endothelial nitric oxide synthases, eNOS) protein in the healing process of rat skin incision. To explore the possibility of its use in forensic practice to infer the time of injury. Methods: the rat skin injury model was established. Immunohistochemical technique (SP) and image analysis were used. To study the changes of iNOS and eNOS protein expression in the injured skin tissue of rats with different injury time (1h-14d) and the effect of different postmortem time on iNOS and eNOS protein. Results: (1) the changes of iNOS protein expression at different time points: no iNOS expression was found in the contralateral tissue of wound at different time points. INOS protein was expressed in posttraumatic keratinocytes, sweat glands, hair follicles, inflammatory cells of skeletal muscle cells and posttraumatic granulation tissue, fibroblasts and vascular endothelial cells in rats. Immunohistochemical staining showed that iNOS protein distributed in the cytoplasm of the above cells as brown granules. 1 granulation tissue: at 6 hours after trauma, macrophages infiltrated near the wound edge, neutrophils began to express iNOS protein and showed weak positive staining. At 12 h, the number of positive staining macrophages and neutrophils increased. The expression of iNOS reached its peak on the 1st day after trauma, and maintained at a high level until 5 days after injury. The strong positive granules were found in the cytoplasm of inflammatory cells, fibroblasts and vascular endothelial cells. At 7 d to 14 d, fibroblasts could still be seen. INOS protein was expressed in neocapillary endothelial cells, but the number of positive cells gradually decreased and the staining degree gradually weakened. 2 epidermis: 1 hour after trauma, microamounts of keratinocytes expressed in wound edge keratinocytes
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：D919
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