法医昆虫种属鉴定的分子生物学方法研究
发布时间：2019-01-27 12:50
【摘要】：目的 本课题旨在探索新的法医昆虫学种属鉴定方法。方法 利用引物设计软件分别对法医常见昆虫的常染色体微卫星区域和线粒体DNA(mtDNA)进行引物设计,使用PCR技术分别对其进行扩增,用聚丙烯酰胺电泳对PCR产物分离、银染。对于微卫星(microsatellite)遗传标记直接进行分型检测。对于线粒体DNA通过银染分离、检测,利用DNA测序仪进行测序分析。用系统进化软件(Mega2.1和PHYLIP3.06)进行法医常见昆虫的亲缘关系分析。结果 采用对线粒体16rsRNA基因进行PCR扩增和DNA测序的方法,我们得到了中国9个地区6种不同法医常见昆
[Abstract]:Objective to explore a new method for identification of forensic entomology species. Methods Primer design software was used to design primers for autosomal microsatellite region and mitochondrial DNA (mtDNA) of common insects in forensic science, PCR technique was used to amplify them, polyacrylamide electrophoresis was used to isolate PCR products and silver staining was used. The genotyping of microsatellite (microsatellite) markers was carried out directly. Mitochondrial DNA was separated and detected by silver staining and sequenced by DNA sequencer. Phylogenetic software (Mega2.1 and PHYLIP3.06) was used to analyze the phylogenetic relationship of common insects in forensic medicine. Results by means of PCR amplification and DNA sequencing of mitochondrial 16rsRNA gene, we obtained 6 kinds of common forensic medicine in 9 regions of China.
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：D919.4
本文编号：2416272
[Abstract]:Objective to explore a new method for identification of forensic entomology species. Methods Primer design software was used to design primers for autosomal microsatellite region and mitochondrial DNA (mtDNA) of common insects in forensic science, PCR technique was used to amplify them, polyacrylamide electrophoresis was used to isolate PCR products and silver staining was used. The genotyping of microsatellite (microsatellite) markers was carried out directly. Mitochondrial DNA was separated and detected by silver staining and sequenced by DNA sequencer. Phylogenetic software (Mega2.1 and PHYLIP3.06) was used to analyze the phylogenetic relationship of common insects in forensic medicine. Results by means of PCR amplification and DNA sequencing of mitochondrial 16rsRNA gene, we obtained 6 kinds of common forensic medicine in 9 regions of China.
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：D919.4
【引证文献】
相关硕士学位论文 前3条
1 白鹏;应用昆虫分子系统学对法医嗜尸性蝇类种属鉴定的研究[D];四川大学;2006年
2 仲雨霞;北京野鸭湖湿地自然保护区甲虫群落多样性及其动态分布的研究[D];首都师范大学;2007年
3 熊枫;应用细胞色素氧化酶亚基I鉴定蝇科常见嗜尸性蝇种[D];中南大学;2012年
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