三重脑震荡鼠轴索、髓鞘及APP、Nogo变化的研究
发布时间：2019-03-30 21:30
【摘要】：目的：观察三重脑震荡鼠轴索、髓鞘变化规律及淀粉样前体蛋白(APP)、轴突生长抑制因子(Nogo)表达变化规律，为运动相关性脑震荡(SRCC)的防治提供一定的理论依据。方法：用金属单摆打击装置复制三重脑震荡大鼠模型，随机分为伤后1、2、4、8、16、24天组共六个损伤组(每组n=8)，同时设正常对照组(n=8)。采用Bielschowsky银染显示轴索及Weil氏染色显示髓鞘，用抗-APP和抗-Nogo多克隆抗体行免疫组化(S-P)法染色。光镜下对脑干轴索APP及Nogo阳性反应的强弱进行定性分析；用图像分析系统分别对银染脑干轴索直径(um)、髓鞘变性程度及海马、顶叶皮质、脑干APP及Nogo阳性反应进行定量分析。统计学处理用单因素方差分析、q检验进行组间多个样本比较及两两比较。结果：1．三重脑震荡损伤后各组均不同程度出现轴索改变，包括轴索肿胀、变形、排列紊乱、断裂甚至轴索收缩球等。对银染轴索直径平均值(urn)进行定量分析，，结果为伤后各组轴索直径均大于对照组，以2、4天组最明显(P＜0.05)。2．髓鞘染色显示伤后各组均不同程度出现髓鞘肿胀、淡染、变性。对其着色程度进行灰度值定量分析，伤后除24天组外，其余各组与对照组相比均有显著性差异(P＜0.05)，以2、4天组最显著。3．APP在神经元及轴索中均见有阳性表达，损伤后先增强，之后降低，再上调至接近正常。其中在脑干轴索APP表现为1天组增强，随后下降，至4天组达低峰，之后逐渐上升恢复正常；在脑干网状结构神经元的1、4、16天组与对照组相比有显著性差异(P＜0.05)；在海马CA3区神经元的1、16天组与对照组相比有显著性差异(P＜0.05)；在顶叶皮质神经元的1、2天组与对照组相比有显著性差异(P＜0.05)。4．Nogo在神经元及轴索纤维中均有阳性表达，伤后表现为先增强然后逐渐恢复，不同部位时间变化规律不同。其中在脑干轴索以4天组最强；在脑干网状结构神经元的1、2、4、8天组与对照组相比有显著性差异(P＜0.05)；在海马CA3区神经元的2、4天组与对照组相比有显著性差异(P＜0.05)；在大脑顶叶皮质神经元的4天组与对照组相比有显著性差异(P＜0.05)。结论：1．三重脑震荡
[Abstract]:Aim: to observe the changes of axons, myelin sheath and (Nogo) expression of amyloid precursor protein (APP), axon growth inhibitor in rats with triple concussion, so as to provide a theoretical basis for the prevention and treatment of exercise-related concussion (SRCC). Methods: the rat model of tricuspid concussion was made by using a metal pendulum striking device. The rats were randomly divided into six injury groups (n = 8) and normal control group (n = 8) on day 1, 2, 4, 8, 16, 24 days after injury. The rats were divided into two groups: the control group (n = 8) and the control group (n = 8). Bielschowsky silver staining and Weil's staining were used to show myelin sheath, and anti-APP and anti-Nogo polyclonal antibodies were used for immunohistochemical staining. The positive reaction of APP and Nogo in brainstem axons was qualitatively analyzed under light microscope. The degree of myelin degeneration and the positive reaction of APP and Nogo in hippocampus, parietal cortex, brainstem and brain stem of silver stained brainstem axons (um),) were quantitatively analyzed by image analysis system. One-way ANOVA and Q-test were used to compare multiple samples and pairwise. Results: 1. Axonal changes, including axonal swelling, deformation, disorder of arrangement, breakage and even axonal contractile ball, were found in all groups after triple concussion injury. The average diameter of silver stained axons (urn) was quantitatively analyzed. The results showed that the axonal diameter of each group was larger than that of the control group after injury, and the most obvious was at the 2nd and 4th day after injury (P < 0.05). 2. Myelin sheath staining showed that myelin sheath swelling appeared in different degrees in all groups after injury. Light dyeing, denaturation. The grayscale analysis showed that there was significant difference between the other groups except the 24-day group after injury (P < 0.05), the most significant expression of app was found in neurons and axons in the 2-and 4-day groups, and the positive expression of app was found in the neurons and axons in the 4-day group, except for the 24-day group (P < 0.05). The damage was enhanced after injury, then decreased, and then up-regulated to close to normal. The APP of brainstem axons was enhanced in 1 day group, then decreased, reached a low peak in 4 days group, then returned to normal gradually, and there was significant difference in 1,4,16 days group compared with the control group (P < 0.05). There was significant difference between the 16-day group and the control group (P < 0.05). The expression of 4.Nogo in neurons and axonal fibers of parietal cortex was significantly higher than that of control group (P < 0.05) at 1 day and 2 days (P < 0.05), and the expression of VEGF in neurons and axonal fibers was enhanced at first and then recovered gradually after injury. The time variation of different parts is different. The brain stem axons were the strongest in the 4-day group, and there was significant difference between the 1, 2, 4 and 8-day groups compared with the control group (P < 0.05). There was a significant difference between the 4-day group and the control group (P < 0.05), and there was a significant difference between the 4-day group and the control group in the 4-day group of hippocampal CA3 neurons (P < 0.05). Triple concussion
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919
本文编号：2450507
[Abstract]:Aim: to observe the changes of axons, myelin sheath and (Nogo) expression of amyloid precursor protein (APP), axon growth inhibitor in rats with triple concussion, so as to provide a theoretical basis for the prevention and treatment of exercise-related concussion (SRCC). Methods: the rat model of tricuspid concussion was made by using a metal pendulum striking device. The rats were randomly divided into six injury groups (n = 8) and normal control group (n = 8) on day 1, 2, 4, 8, 16, 24 days after injury. The rats were divided into two groups: the control group (n = 8) and the control group (n = 8). Bielschowsky silver staining and Weil's staining were used to show myelin sheath, and anti-APP and anti-Nogo polyclonal antibodies were used for immunohistochemical staining. The positive reaction of APP and Nogo in brainstem axons was qualitatively analyzed under light microscope. The degree of myelin degeneration and the positive reaction of APP and Nogo in hippocampus, parietal cortex, brainstem and brain stem of silver stained brainstem axons (um),) were quantitatively analyzed by image analysis system. One-way ANOVA and Q-test were used to compare multiple samples and pairwise. Results: 1. Axonal changes, including axonal swelling, deformation, disorder of arrangement, breakage and even axonal contractile ball, were found in all groups after triple concussion injury. The average diameter of silver stained axons (urn) was quantitatively analyzed. The results showed that the axonal diameter of each group was larger than that of the control group after injury, and the most obvious was at the 2nd and 4th day after injury (P < 0.05). 2. Myelin sheath staining showed that myelin sheath swelling appeared in different degrees in all groups after injury. Light dyeing, denaturation. The grayscale analysis showed that there was significant difference between the other groups except the 24-day group after injury (P < 0.05), the most significant expression of app was found in neurons and axons in the 2-and 4-day groups, and the positive expression of app was found in the neurons and axons in the 4-day group, except for the 24-day group (P < 0.05). The damage was enhanced after injury, then decreased, and then up-regulated to close to normal. The APP of brainstem axons was enhanced in 1 day group, then decreased, reached a low peak in 4 days group, then returned to normal gradually, and there was significant difference in 1,4,16 days group compared with the control group (P < 0.05). There was significant difference between the 16-day group and the control group (P < 0.05). The expression of 4.Nogo in neurons and axonal fibers of parietal cortex was significantly higher than that of control group (P < 0.05) at 1 day and 2 days (P < 0.05), and the expression of VEGF in neurons and axonal fibers was enhanced at first and then recovered gradually after injury. The time variation of different parts is different. The brain stem axons were the strongest in the 4-day group, and there was significant difference between the 1, 2, 4 and 8-day groups compared with the control group (P < 0.05). There was a significant difference between the 4-day group and the control group (P < 0.05), and there was a significant difference between the 4-day group and the control group in the 4-day group of hippocampal CA3 neurons (P < 0.05). Triple concussion
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919
【参考文献】
相关期刊论文 前5条
1 于晓军,肖飞,吴家櫦,吴梅筠;大鼠闭合性脑损伤酯化银和白蛋白组织化学研究[J];法医学杂志;2001年02期
2 朱金龙,朱少华,任亮,刘良,周亦武,陈虎,邓伟年;大鼠弥散性轴索损伤后β-APP的表达[J];法医学杂志;2005年03期
3 李宗平;颅脑损伤后β-APP及其产物在脑组织中的变化及临床意义[J];国外医学(神经病学神经外科学分册);2003年01期
4 于建云,李树华,韩晓华,许冰莹,林海英,赵旭东;脑震荡鼠细胞组织病理学研究[J];昆明医学院学报;2003年02期
5 刘雁,石尚金,董为伟;脑梗死后自发性高血压大鼠脑内Nogo-A mRNA的表达[J];中国临床康复;2003年05期
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