SCD心脏ANP与BNP的免疫组织化学研究

发布时间：2019-04-19 03:14

【摘要】：背景及目的:SCD 检验鉴定是法医病理学中的常见问题之一。心肌缺血被认为是 SCD 的重要原因,但 SCD 肉眼检查及常规组织病理学检查心肌梗死灶检出率很低,寻找可在心肌组织上灵敏表达的早期心缺血指标,成为 SCD 研究课题的重点及难点。但对可导致 SCD 发生的非缺血因素以及 SCD 发生机制却研究很少。ANP、BNP 属心脏的神经内分泌激素,对循环血量、电解质、血管等具有调节作用,其血浆水平能反映心脏功能状况,在可导致 SCD 的多因素(缺血性和非缺血性)中,利钠肽均具有良好相关性。本实验利用尸检心肌标本,应用免疫组织化学方法检测 ANP、BNP 在 SCD 组心肌、非 SCD 心肌、非 SD 正常心肌中的表达情况,初步探讨 NP 在法医学诊断 SCD 的价值。 方法:1.选材: 选取法医教研室 2000 年至 2004 年尸检心脏标本36 例,其中男性 21 人,女性 15 人,平均年龄 44.9 岁,(具体年龄构成见表 1)按以下标准分为 3 组:(1).SCD 组 12 例(A 组),其中冠状动脉硬化性心脏病(冠状动脉Ⅲ°以上狭窄)未伴明显梗死灶 4 例,心肌病 4 例,心肌炎 4 例,以上均经肉眼和显微镜同时证实,符合诊断标准,其它系统没有检见严重器质性疾病,法医病理学诊断为 SCD;(2)非 SCD组 12 例(B 组),其中脑出血 4 例, 肺栓塞 4 例,急性腹膜炎 4 例,其中在肉眼和显微镜中心脏均未检见较重器质性疾病.在(1)(2)两组,均为平素身体貌似健康,突然发病所致,从发病到死亡在 24 小时以内.(3)非 SD正常心肌组 12 例(C 组),即因心脏外疾病死亡,从发病到死亡在 48 小
[Abstract]:Background & objective: SCD examination and identification is one of the common problems in forensic pathology. Myocardial ischemia is considered to be an important cause of SCD, but the detection rate of myocardial infarction by SCD naked eye examination and routine histopathological examination is very low, so it is necessary to search for early myocardial ischemia markers that can be sensitively expressed in myocardial tissue. It has become the focus and difficulty of SCD research. However, there are few studies on the non-ischemic factors and the mechanism of SCD. BNP, a neuroendocrine hormone of the heart, can regulate the circulation blood volume, electrolytes and blood vessels, and its plasma level can reflect the state of the heart function, and its plasma level can reflect the state of the heart function, and its plasma level can reflect the state of the heart function. Natriuretic peptides are well correlated in the multiple factors (ischemic and non-ischemic) that can lead to SCD. In this study, the expression of ANP,BNP in myocardium of SCD group, non-SCD myocardium and normal myocardium of non-SD was detected by immunohistochemical method, and the value of NP in forensic diagnosis of SCD was discussed. Methods: 1. Material selection: from 2000 to 2004, 36 autopsy heart specimens were selected from the Forensic Laboratory, including 21 males and 15 females, with an average age of 44.9 years. (see Table 1) divided into 3 groups according to the following criteria: (1) 12 cases in). SCD group (group A), including 4 cases of coronary sclerosing heart disease (stenosis of coronary artery above 鈪，

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