五种常见嗜尸性蝇类的分子鉴定
发布时间：2019-05-29 17:08
【摘要】：为了解决法医学人员对于嗜尸性蝇类的鉴定难题,对中山市、广州市及西安市5种常见嗜尸性蝇类共17个样本,对其线粒体COⅠ基因的348bp大小片段进行了RFLP和DNA序列分析,采用ABI377测序仪测序,DNASTAR软件预测限制性位点,DdeⅠ,DraⅠ和HinfⅠ3种限制性内切酶消化,非变性聚丙烯酰胺凝胶电泳检测酶切结果,MEGA3.0软件包进行序列分析和构建系统发育树。结果表明:采用mtDNA扩增结合银染技术和DNA序列分析,均可以方便快捷地进行上述三地5种常见嗜尸性蝇类的种类鉴定。本文结果为法医昆虫学中嗜尸性昆虫DNA鉴定数据库的建立提供了数据资料。
[Abstract]:In order to solve the difficult problem of forensic personnel in the identification of cadaveric flies, 17 common cadaveric flies were collected from Zhongshan, Guangzhou and Xi'an, and the 348bp fragments of CO 鈪，
本文编号：2488076
[Abstract]:In order to solve the difficult problem of forensic personnel in the identification of cadaveric flies, 17 common cadaveric flies were collected from Zhongshan, Guangzhou and Xi'an, and the 348bp fragments of CO 鈪，
本文编号：2488076
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