李渡酒窖土遗址中微生物种群的鉴定与分析

发布时间：2018-05-23 23:09

本文选题：李渡酒窖土遗址 + 微生物种群　；参考：《微生物学通报》2017年01期

【摘要】：【目的】对李渡酒窖土遗址的微生物病害进行防护和治理,对土遗址的微生物种群进行鉴定与分析。【方法】从土遗址现场采集具代表性的微生物病害样品3份,构建其16S r RNA基因和18S r RNA-ITS基因间隔区的克隆文库,测序后利用MEGA 3.0和Glustal W 1.8软件对测序结果进行分析,并构建系统发育树。【结果】共获得72个有效的细菌16S r RNA基因序列,归属于3个门11个属;80个有效的真菌18S r RNA-ITS基因间隔序列归属于1个门7个属。细菌的优势菌为盐单胞菌属(Halomonas sp.)、甲基盐单胞菌属(Methylohalomonas sp.)和乳杆菌属(Lactobacillus sp.)。真菌的优势菌为:Cyphellophora sp.、毕赤酵母属(Pichia sp.)、外瓶霉属(Exophiala sp.)和瓶霉属(Phialophora sp.)。【结论】实验分析获得的优势细菌多具有耐盐碱的特性,这与酒窖土遗址的现场环境相一致;真菌中的优势真菌主要为丝状真菌和酵母。通过对土遗址内主要微生物种群的鉴定与分析,确定出了可能对土遗址造成严重破坏作用的微生物,为后期加固材料的耐微生物降解性研究提供了依据,该研究结果对李渡酒窖土遗址的保护具有重要意义。
[Abstract]:[objective] to protect and control the microbial diseases in Lidujiu Jiujiao soil site, and to identify and analyze the microbial population of the soil site. [methods] three representative samples of microbial diseases were collected from the site. The 16s r RNA gene and 18s r RNA-ITS gene spacer library were constructed, the sequencing results were analyzed by MEGA 3.0 and Glustal W1.8 software, and the phylogenetic tree was constructed. [results] 72 effective 16s r RNA gene sequences were obtained. They belong to 11 genera in 3 phylum and 7 genera in 1 phylum, and 80 effective spacer sequences of 18s r RNA-ITS gene belong to 7 genera of 1 phylum. The dominant bacteria were Halomonas sp. and Methylohalomonas sp. And Lactobacillus sp. The dominant fungi were Cyphellophora sp., Pichia sp. and Exophiala sp. [conclusion] the dominant bacteria obtained from the experimental analysis are saline-alkali tolerant, which is consistent with the site environment of the wine cellar soil site, and the dominant fungi in the fungi are mainly filamentous fungi and yeast. Through the identification and analysis of the main microbial populations in the earthen sites, the microbes which may cause serious damage to the earthen sites are identified, which provide the basis for the research on the biodegradability of the reinforcement materials in the later stage. The results of this study are of great significance to the protection of the Lidu Jiujiao soil site.
【作者单位】：南开大学生命科学学院微生物系分子微生物学与技术教育部重点实验室;中国文化遗产研究院;浙江大学化学系;
【基金】：李渡烧酒作坊遗址本体防风化保护试验与防渗保护方案设计项目~~
【分类号】：K878;Q93

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