固相萃取C4技术在文物蛋白胶料气相色谱前处理中的应用

发布时间：2019-03-05 17:44

【摘要】：在引入固相萃取(SPE)C4前处理技术的基础上,首次采用气相色谱质谱联用(GC-MS)和离子色谱(ICS)技术定性、定量化评估了SPE C4柱对常见文物保存环境中可溶盐离子的排除效果,进一步探讨了该技术对氨基酸分析结果的影响、保护色谱柱的效果及对文物样品测试的适用性。结果表明,SPE C4技术能有效排除Na~+,K~+,Mg~(2+),Ca~(2+),Cl~-,NO_3~-,SO_4~(2-)等常见可溶盐离子,平均洗脱率(脱盐率)达99.99%;添加30 g/L NaCl的猪皮胶经SPE C4处理后测得的色谱图出峰完整,未检测到Na~+和Cl~-,11种氨基酸含量测量结果的平均相对误差约5%。文物样品氨基酸色谱图基线平稳、分离良好。因此,SPE C4技术适用于中国古代蛋白胶料气相色谱分析的前处理中,对分析鉴定我国古代文物蛋白胶料具有极高的实用价值。
[Abstract]:Based on the introduction of solid-phase extraction (SPE)-C _ 4) pretreatment technology, gas chromatography-mass spectrometry (GC-MS) and ion chromatography-(ICS) were used for the first time. The effect of SPE C4 column on the removal of soluble salt ions in the preservation environment of common cultural relics was quantitatively evaluated. The influence of the technique on amino acid analysis results, the effect of protection column and the applicability of the technique to the determination of cultural relics samples were further discussed. The results showed that SPE C4 technology could effectively eliminate the common soluble salt ions such as Na~, K ~, Mg~ (2), Ca~ (2), Cl~-,NO_3~-,SO_4~ (2 -), and the average desalination rate (desalination rate) was 99.99%. After being treated with 30 g / L NaCl, the chromatogram showed a complete peak, and the average relative error of Na~ and Cl~-,11 amino acid content measurement results was about 5%. The baseline of amino acid chromatogram of cultural relics is stable and the separation is good. Therefore, SPE-C4 technology is suitable for the pre-treatment of Chinese ancient protein compound by gas chromatography, and it is of great practical value for the analysis and identification of protein compound of ancient cultural relics in China.
【作者单位】：西北大学文化遗产学院;文化遗产研究与保护技术教育部重点实验室;
【基金】：国家自然科学基金(21175104) 陕西省教育厅基金(13JZ045)资助
【分类号】：K854;O657.71

【相似文献】