有机垃圾热解炭对紫色土细菌群落结构的影响

发布时间：2017-12-28 05:17

本文关键词：有机垃圾热解炭对紫色土细菌群落结构的影响　出处：《中国环境科学》2017年02期 　论文类型：期刊论文

【摘要】：以700℃热解制得城市有机垃圾(OFMSW)生物炭,为研究该生物炭对氮素缺乏的紫色土有机质、氮营养元素以及微生物群落结构的影响,设计了为期12个月的土壤盆栽培养试验.试验共设置生物炭添加量分别为0%、1%、3%和5%(w/w)的4个处理.采用高通量测序技术分析土壤的微生物群落结构.试验表明:3%和5%的生物炭添加量显著提高了土壤有机质和全氮含量,同时显著降低了紫色土中细菌的α-多样性;主成分分析显示,0%与1%添加量处理的细菌群落组成相似,3%与5%添加量处理的细菌群落组成相似.Illumina测序从24个土样中鉴定出了42门细菌,其中主要的6个菌门为变形菌门、酸杆菌门、拟杆菌门、放线菌门、绿弯菌门和芽单胞菌门,它们序列数占序列总数的83.7%~94.3%.各菌门对不同的生物炭添加量响应不同.从24个土壤样本中鉴定出642个菌属,相对丰度大于1%的有105个.部分主要菌属对3%和5%的生物炭添加量的响应与0%和1%的不同.
[Abstract]:At 700 C pyrolysis city organic waste (OFMSW) to study the biological carbon, biological activated carbon to nitrogen deficiency of soil organic matter, nitrogen nutrient and microbial community structure, soil pot designed for a period of 12 months of training test. Test sets of biochar additive amount were 0%, 1% 3% and 5% (w/w) 4. Analysis of soil microbial community structure by high-throughput sequencing. The results showed that 3% and 5% of biochar addition significantly increased soil organic matter and total nitrogen content, while significantly reducing bacteria in purple soil of alpha diversity; principal component analysis showed that 0% and 1% add the amount of the bacterial community composition similar to that of 3% and 5% added amount of bacterial community composition similar to.Illumina sequencing from 24 soil samples identified 42 bacteria, 6 of which were the main bacterial phyla Proteobacteria and acidobacteria, Bacteroides The door, Actinobacteria, Chloroflexi and gemmatimonadetes, accounting for 83.7%~94.3%. of the total number of the BAC sequence to the sequence number of different bacteria they added. Different responses from 24 soil samples identified 642 species, relative abundance of more than 1%. There are 105 main parts of bacteria 3% and 5% of biochar on the amount of response and 0% and 1%.
【作者单位】：重庆大学三峡库区生态环境教育部重点实验室;
【基金】：重庆市自然科学基金重点项目(2011BA7020)
【分类号】：S154.3;X705
【正文快照】： 紫色土是我国特有的土壤资源,分布面积有2000多万hm2[1].由于成土快、发育浅、透气性好、矿物营养丰富、酸碱适中,紫色土中的好养微生物十分活跃,造成土壤有机质(SOM)的矿化势和矿化率均较高,加之垦植率高,腐蚀严重,导致SOM和土壤全氮(TN)含量均较低[1].与此同时生物炭作为一

【参考文献】