耐重金属的植物促生芽孢杆菌筛选及其强化香蒲去除Cd的作用

发布时间：2018-01-01 19:24

本文关键词：耐重金属的植物促生芽孢杆菌筛选及其强化香蒲去除Cd的作用　出处：《农业环境科学学报》2017年11期 　论文类型：期刊论文

【摘要】：为探讨细菌对水生植物修复水体重金属污染的影响,从南京某矿区废弃地的湿地植物柳树(Salix babylonica)、芦苇(Phragmites australis)、香蒲(Typha latifolia)根际和根内分离筛选到8株芽孢杆菌,这些芽孢杆菌都具有产铁载体、吲哚乙酸、多糖和淀粉酶活性,且对重金属的抗性较强,对温度、pH和盐浓度具有一定的耐受性。采用水培试验研究了Cd胁迫下耐重金属植物促生芽孢杆菌强化狭叶香蒲(T.angustifolia)吸收积累Cd的作用,结果表明,在2.5 mg·L~(-1)Cd~(2+)的条件下接种芽孢杆菌处理能够促进香蒲生长和组织中Cd含量增加。其中Bacillus megaterium P24和P37促进香蒲根和地上部干重显著增加40.0%~46.4%,B.amyloliquefaciens P29能使地上部Cd含量和总吸收量显著增加31.1%和63.5%;溶液中Cd去除率最高可达79.5%。研究表明,耐重金属芽孢杆菌能够促进香蒲生长、增加香蒲Cd含量,从而增加香蒲Cd总吸收量,达到去除水中Cd污染的作用,具有应用于水体重金属污染修复的潜力。
[Abstract]:In order to investigate the effect of bacteria on the remediation of heavy metal pollution by aquatic plants, Salix babylonica, a wetland plant in an abandoned land of a mining area in Nanjing, was studied. Eight strains of Bacillus spp. Were isolated from rhizosphere and root of Phragmites australis, Typha latifolia. All of these bacilli have iron-producing carriers, indoleacetic acid, polysaccharides and amylase activities, and are resistant to heavy metals and temperature. The pH and salt concentration of T. angustifolia (T. angustifolia) enhanced by Bacillus spp., a heavy metal tolerant plant under CD stress, was studied by hydroponics. Absorption and accumulation of CD. It turns out. At 2.5 mg 路L ~ (-1). Inoculation with Bacillus spp can promote the growth of Typha and the increase of CD content in tissues. Among them, Bacillus megaterium can increase CD content. P24 and P37 increased the dry weight of Typha root and aboveground by 46.4%. B.amyloliquefaciens P29 significantly increased CD content and total uptake of CD in shoot by 31.1% and 63.5% respectively. The highest removal rate of CD in the solution was 79.5%. The results showed that the heavy metal tolerant bacillus could promote the growth of Typha cattails and increase the CD content of Typha cattails, thus increasing the total CD absorption of Typha cattails. In order to remove CD pollution in water, it has the potential to be applied to the remediation of heavy metal pollution in water.
【作者单位】：农业部农业环境微生物重点实验室南京农业大学生命科学学院;
【基金】：国家自然科学基金项目(41471273)~~
【分类号】：X172
【正文快照】： 近年来随着经济的迅速发展,矿业开发、工业生产、城市生活产生的废水排入水体,使水质逐渐恶化,其中重金属是主要的污染物。大量重金属的存在给水体生物造成了严重危害,甚至威胁着人体健康[1-3]。因此,水体重金属污染已经成为国内外亟需解决的环境问题。水生植物修复技术是利用

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