施粪肥土壤中抗生素的提取条件优化及残留特征
发布时间：2018-01-01 22:03
本文关键词：施粪肥土壤中抗生素的提取条件优化及残留特征 出处：《环境工程学报》2017年11期 论文类型：期刊论文
【摘要】：为确立高效的土壤抗生素提取条件,揭示施用粪肥土壤中抗生素的残留水平,分别采集江苏省常州市某养猪场新鲜猪粪样品6份、施用粪肥及无机肥水稻土壤样品各13份,对土壤抗生素提取液配比与超声提取时间进行筛选,利用高效液相色谱-串联质谱(HPLC-MS/MS)对生猪粪便和土壤中的四大类(四环素类、磺胺类、喹诺酮类和大环内脂类)15种抗生素含量进行检测分析。结果表明,最优提取条件选择为:提取液配比为甲醇:EDTA=1∶1,超声提取时间为10 min,四环素类抗生素的加标回收率为73.2%~93.4%,磺胺类为81.2%~101.1%,喹诺酮类为106.3%~110.8%。该养猪场猪粪与施粪肥土壤中均未检测到大环内脂类抗生素,其余各类抗生素平均残留量从高到低排序分别为:猪粪中四环素类磺胺类喹诺酮类;施粪肥土壤中四环素类喹诺酮类磺胺类,四环素类是最主要的抗生素污染物,残留量分别为猪粪中1.9~12.5 mg·kg-1、施粪肥土壤中23.9~212.4μg·kg-1;施无机肥土壤中未检测出抗生素,施粪肥土壤中的抗生素来源于生猪粪便。
[Abstract]:The extraction conditions for the establishment of effective antibiotics in soil, revealing the level of residual antibiotics application of manure in soil were collected in Jiangsu city of Changzhou Province, a pig farm fresh pig manure and 6 samples, using manure and rice inorganic fertilizer soil samples of 13 copies each, on soil antibiotic ratio and extraction time were screened by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) of pig manure and soil in four categories (tetracyclines, sulfonamides, quinolones and macrolides) were detected and analyzed the content of 15 kinds of antibiotics. The results showed that the optimal extraction conditions were: extraction ratio of methanol to EDTA=1: 1, ultrasonic extraction time was 10 Min the recovery of tetracycline antibiotics, sulfa rate is 73.2%~93.4%, 81.2%~101.1%, 106.3%~110.8%. of the quinolones pig farm pig manure and manure in soil were not detected in macrolide resistance The other kinds of vitamins, antibiotics average residues from high to low ranking were: tetracycline sulfa quinolones in pig manure; application of tetracycline quinolone sulfa manure in the soil, tetracycline is the main pollutant of antibiotic residues in pig manure, respectively 1.9~ 12.5 mg - kg-1, manure in soil 23.9~212.4 g kg-1; soil inorganic fertilizer did not detect antibiotics, manure in soil from pig feces. Antibiotics
【作者单位】： 常州大学环境工程与安全学院;土壤与农业可持续发展国家重点实验室中国科学院南京土壤研究所;
【基金】：国家自然科学基金资助项目(41371256) “十二五”国家科技支撑计划项目(2015BAC02B02-01)
【分类号】：X53;X833
【正文快照】： 2.土壤与农业可持续发展国家重点实验室,中国科学院南京土壤研究所,南京210008自1929年青霉素发现以来,抗生素就被用于治疗人和动物的疾病,或向动物饲料中添加兽用抗生素提高动物的生长率和饲料的转化率[1]。抗生素在环境中的残留已引起了人们极大的关注[2-3],进入机体的抗生，
本文编号：1366375
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