基于垂直折流式生物膜反应器加速三氯酚与硫酸盐生物降解研究

发布时间：2018-01-03 03:13

本文关键词：基于垂直折流式生物膜反应器加速三氯酚与硫酸盐生物降解研究　出处：《上海师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：随着现代化工业的发展,印染厂、造纸厂常将硫酸盐与氯酚类化合物作为工艺的辅助材料和化工原料,使此类工业废水中不仅含有高浓度的氯酚类化合物,还含有大量的硫酸盐。一方面,氯酚类化合物是一种具有“致癌、致畸、致突变”特性的难降解有机物,进入环境后将对人类的健康和生态环境造成严重的危害,另一方面,由于硫酸盐的存在将会导致含氯酚类化合物的废水在处理上受到影响。而现有处理氯酚类废水和硫酸盐废水的方法主要采用生物处理技术,生物处理技术具有即经济又易管理等优点。本研究采用摇瓶作为生物反应器,通过控制摇瓶开口时间,了解厌氧与缺氧环境下2,4,6-三氯酚(2,4,6-TCP)生物降解情况,进一步采用垂直折流式生物膜反应器处理2,4,6-TCP及探索硫酸盐存在下,2,4,6-TCP与硫酸盐生物降解过程中对外加电子供体的关系,并探讨最佳碳源量的选择,结果表明:1、通过摇瓶实验发现,当在厌氧时,2,4,6-TCP主要以还原脱氯的形式将多氯酚转换成低氯酚,而低氯酚在厌氧环境下很难进一步生物降解,而在有一定溶解氧的情况时,可迅速达到生物降解。因此,采用先厌氧后好氧的形式处理2,4,6-TCP能有效提高2,4,6-TCP生物降解。2、在2,4,6-TCP还原脱氯过程所产生的一级中间产物2,4-DCP对2,4,6-TCP生物降解过程存在一定的抑制作用,二级产物4-CP对一级产物2,4-DCP生物降解过程存在一定的抑制作用,二级产物4-CP对2,4,6-TCP生物过程刚影响不大,因此对于2,4,6-TCP的生物脱氯反应过程中,各中间产物是逐级影响上一级,而非跨级影响,可通过加速4-CP的生物降解来逐级提高2,4,6-TCP的生物降解速率。3、通过加入易降解的丙酸钠、乙酸钠和葡萄糖作为外加碳源进行实验可得,丙酸钠、乙酸钠和葡萄糖均可作为有效提高2,4,6-TCP还原脱氯的外加电子供体,而对于硫酸盐还原过程中丙酸钠作为电子供体要高于乙酸钠和葡萄糖作为电子供体进行生物处理。另一方面,利用葡萄糖作为电子供体时2,4,6-TCP转化成2,4-DCP的转化率最大,而转化成4-MCP的转化率最小,利用乙酸钠作为电子供体时2,4,6-TCP转化成2,4-DCP的转化率最小,而转化成4-MCP的转化率最大。4、随着增加外加电子供体的量,可以有效地提高2,4,6-TCP和硫酸盐生物降解速率;同时生物降解2,4,6-TCP与硫酸盐时,2,4,6-TCP还原脱氯过程与硫酸盐还原过程对电子存在着竞争关系,2,4,6-三氯酚还原脱氯对电子的竞争能力强于硫酸盐还原过程对电子的竞争能力;而对于本实验采用的垂直折流式生物膜反应器降解50μM 2,4,6-三氯酚和0.28 mM的硫酸盐混合废水时,最佳碳源量还可控制在1.08 mmol左右。
[Abstract]:With the development of modern industry, sulphate and chlorophenol compounds are often used as auxiliary materials and chemical raw materials in printing and dyeing factories and paper mills, which make such industrial wastewater contain not only high concentration of chlorophenol compounds. On the one hand, chlorophenols are refractory organic compounds with the characteristics of "carcinogenesis, teratogenicity, mutagenicity". Entering the environment will cause serious harm to human health and ecological environment, on the other hand. Due to the presence of sulfate, the treatment of wastewater containing chlorophenols will be affected, while the existing treatment methods of chlorophenol wastewater and sulfate wastewater are mainly biological treatment technology. The biological treatment technology is economical and easy to manage. In this study, the shaking flask was used as a bioreactor. By controlling the opening time of the shaking flask, we could understand the 2 ~ (4) O ~ (4) -trichlorophenol ~ (2 +) -trichlorophenol 2 ~ (4) in anaerobic and anoxic environment. The biodegradation of 6-TCPwas further treated by vertical biofilm biofilm reactor, and the treatment of 2-TCP-6-TCP in the presence of sulphate was explored. The relationship between 6-TCP and the external electron donor in the process of sulfate biodegradation and the selection of the optimum carbon source were discussed. 6-TCP mainly converts polychlorophenol to low chlorophenol in the form of reductive dechlorination, which is difficult to biodegrade in anaerobic environment, but when there is a certain amount of dissolved oxygen. Biodegradation can be achieved rapidly. Therefore, the treatment of 2n4N 6-TCP in the form of first anaerobic and then aerobic treatment can effectively increase the biodegradation of 2O4C6TCP, at 2O4. The first intermediate product 2n4-DCP produced by the reduction dechlorination process of 6-TCP has a certain inhibitory effect on the biodegradation process of 2n4N 6-TCP, and the second product 4-CP has a certain effect on the first product 2. 4-DCP biodegradation process has a certain inhibitory effect, the secondary product 4-CP has little effect on the biological process of 2Con 4N 6-TCP, so it has little effect on 2O4. In the biological dechlorination reaction of 6-TCP, each intermediate product has a step by step effect, not a cross-stage effect, which can be increased by accelerating the biodegradation of 4-CP step by step. The biodegradation rate of 6-TCP. 3 was obtained by adding readily degradable sodium propionate, sodium acetate and glucose as additional carbon source. 6-TCP reductive dechlorination of the external electron donor, but in the sulfate reduction process, sodium propionate as an electronic donor is higher than sodium acetate and glucose as electronic donor biological treatment. On the other hand. When glucose was used as electron donor, the conversion rate of 2o 4N 6-TCP was the highest, and the conversion rate of conversion to 4-MCP was the lowest. When sodium acetate was used as electron donor, the conversion rate was 2H4. The conversion rate of 6-TCP to 2n4-DCP was the smallest, while the conversion rate of 4-MCP was the largest. With the increase of the amount of additional electron donor, the conversion rate of 6-TCP was the lowest and the conversion of 4-MCP was the largest. 6-TCP and sulfate biodegradation rate; At the same time, there is a competitive relationship between the reduction dechlorination process and the sulfate reduction process. The competitive power of 6- trichlorophenol to electrons is stronger than that of sulfate reduction. The vertical baffled biofilm reactor was used to degrade 50 渭 M 2N 4 trichlorophenol and 0.28 mm sulphate mixed wastewater. The optimum carbon source can also be controlled at about 1. 08 mmol.
【学位授予单位】：上海师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：X703

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