石油降解菌群的构建及其对混合烃的降解特性

发布时间：2018-01-05 13:36

本文关键词：石油降解菌群的构建及其对混合烃的降解特性　出处：《农业环境科学学报》2017年03期 　论文类型：期刊论文

【摘要】：经富集、分离、纯化,并经选择性培养基的筛选和对总石油烃(TPH)降解能力的测试,从大庆油田石油污染土壤中获得6株分别具有环烷烃、直链烷烃和芳烃降解能力的菌株B_1、B_2、B_3、B_6、B_7和B_9。经16S r RNA基因序列比对,菌株B_1、B_2、B_6和B_7分别属于嗜氢菌属(Hydrogenophaga sp.)、苯基杆菌属(Phenylobacterium sp.)、鞘脂菌属(Sphingobium sp.)和芽胞杆菌属(Bacillus sp.),B_3和B_9属于节杆菌属(Arthrobacter sp.)。通过对接种比例、接种量和生长条件的优化,构建了微生物降解菌群,并采用该菌群进行混合烃污染土壤的修复。结果表明,该菌群对三类烃的去除均具有明显的促进作用。经过40 d的修复,环十二烷去除率达74.5%;100 d后,正十六烷和芘的去除率分别达56.9%和60.4%。前10 d修复过程中,各污染物降解速率最大,之后则逐渐降低,与土壤微生物多样性、数量以及活性的变化趋势相似。
[Abstract]:Purified by enrichment, separation, screening, and by the selective medium and the total petroleum hydrocarbons (TPH) biodegradation test, 6 strains were obtained with naphthenic oil from Daqing oil polluted soil, strain B_1, and the degradation ability of B_2 alkanes aromatic hydrocarbons, B_3, B_6, B_7 and B_9. by 16S R RNA comparison of gene sequence of strain B_1, B_2, B_6 and B_7 respectively belong to hydrogen bacteria (Hydrogenophaga, sp.) - (Phenylobacterium sp.), Bacillus sp. (Sphingobium sp.) sphingolipids and Bacillus (Bacillus sp.), B_3 and B_9 belong to the genus of rod (Arthrobacter sp.). The proportion of inoculation optimization, inoculation amount and growth conditions, the construction of microbial degradation bacteria, and the bacteria were repaired with mixed hydrocarbon contaminated soil. The results showed that the flora of three kinds of hydrocarbon removal had obvious promoting effect. After the restoration of 40 d, twelve ring alkane removal rate was 74.5%; After 100 D, sixteen alkanes and pyrene removal rate reached 56.9% 60.4%. and 10 d before the repair process, the degradation rate is highest, then gradually decreased, and soil microbial diversity, the number and activity of a similar trend.

【作者单位】：北方民族大学生物科学与工程学院;中国科学院沈阳应用生态研究所;
【基金】：国家水体污染控制与治理科技重大专项(2013ZX07202-007) 宁夏高等学校科研项目(NGY2016153) 北方民族大学重点科研项目(2015KJ34);北方民族大学科研项目(2016skky07)~~
【分类号】：X172;X53
【正文快照】： 在各种石油污染土壤修复方法中,生物修复技术以其操作简单、处理费用低、环境影响小等优点而受到人们的重视。多项研究证明生物修复在脂肪烃和芳香烃的降解中起着重要作用[1-3]。Project supported:Water Pollution Control and Management Key Project of Science and Techno

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