洱海沙坪湾湖滨带不同植被带中细菌分布特征

发布时间：2018-01-25 21:29

本文关键词： 洱海湖滨带植被带细菌群落分布特征高通量测序　出处：《环境科学研究》2017年05期 　论文类型：期刊论文

【摘要】：湖滨带是湖泊保护的重要屏障,其不同植被带中的细菌对入湖污染物降解起重要作用.为研究不同植被带中的细菌分布,以洱海沙坪湾湖滨带为研究对象,采用Illumina Hi Seq高通量测序方法,对其中AG(乔草植被带)、EP(挺水植被带)、SP(沉水植被带)和UV(无植被带)的土壤或沉积物中的细菌多样性和群落结构进行研究.结果表明:湖滨带植被带中细菌种类丰富,共有54个门、106个纲、192个目、334个科和503个属被检测到,细菌的OTUs从高到低依次为EP(3 162个)、AG(2 926个)、UV(2 709个)和SP(2 427个),Shannon-Wiener多样性指数从高到低依次为EP(10.14)、AG(9.75)、SP(9.44)和UV(9.26);湖滨带植被带中主要细菌类群是变形菌门(Proteobacteria)(AG,48.55%;EP,47.83%;SP,40.36%;UV,44.00%)、酸杆菌门(Acidobacteria)(AG,19.30%;EP,5.31%;SP,5.67%;UV,6.30%)、厚壁菌门(Firmicutes)(AG,6.01%;EP,8.24%;SP,3.96%;UV,13.13%)和绿弯菌门(Chloroflexi)(AG,3.98%;EP,8.91%;SP,8.95%;UV,7.59%),主要类群在不同植被带中占比不同;植被带类型对细菌的群落结构特征影响不大,但对细菌种群的多样性影响较大.典型对应分析(CCA)结果显示,w(TP)、pH和w(OM)(OM为有机质)是影响不同植被带细菌种群结构的主要环境因子.
[Abstract]:The lakeshore zone is an important barrier for lake protection, and the bacteria in different vegetation zones play an important role in the degradation of pollutants into the lake. In order to study the distribution of bacteria in different vegetation zones, the Shaping Bay lakeside zone in Erhai Lake is taken as the research object. The high throughput sequencing method of Illumina Hi Seq was used to analyze the Illumina (Arbor vegetation belt). The bacterial diversity and community structure in soil or sediment of SP (submerged vegetation zone) and UV (non-vegetative zone) were studied. The results showed that the species of bacteria in the lakeside zone were rich, with 54 phyla. A total of 106 classes, 192 orders, 334 families and 503 genera were detected, and the OTUs of bacteria was EP(3 162 and AGN 2 926 from high to low. UV(2 (709) and SP(2 (427) Shannon-Wiener diversity index from high to low was EPX 10.14 + AG9.75). SPV 9.44) and UV 9.26; The main bacteria group in the lakeshore vegetation zone is Proteobacteria amurensis 48.55; EPN 47.83; SPN 40.36; Acidobacteria acidobacteriae (Acidobacteria), Acidobacteria (Acidobacteria), Acidobacteria (Acidobacteria); EP5. 31; SPN 5.67; UV6.30%, the phylum phylum Firmicutesus is 6.01%; EP8. 24; SPN 3.96; UV 13.13) and Chloroflexiaceae 3.98; EP8. 91; SPN 8.95; The proportion of main groups in different vegetation zones is different. The type of vegetation belt had little effect on the community structure of bacteria, but had a great effect on the diversity of bacterial population. Ph and OM are the main environmental factors affecting the population structure of bacteria in different vegetation zones.
【作者单位】：西安建筑科技大学环境与市政工程学院;中国环境科学研究院国家环境保护湖泊污染控制重点实验室湖泊水污染治理与生态修复技术国家工程实验室;中国环境科学研究院水污染控制技术研究中心;
【基金】：国家水体污染控制与治理科技重大专项(2012ZX07105-002)
【分类号】：X524
【正文快照】： 湖滨带是湖泊最高水位线与最低水位线之间的水陆交错带,其生态系统中细菌数量众多且种类丰富,对其中物质的降解、转化起着关键的控制作用,并且不同种类细菌所起的作用也不同[1],是研究湖泊生物地球化学循环的热点[2].近年来,对于微生物尤其是细菌群落组成、多样性与淡水湖泊非

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