小球藻、活性污泥对分散蓝2BLN的去除研究及藻菌共生体系构建

发布时间：2018-02-02 19:02

本文关键词： 小球藻活性污泥染料藻菌共生体系　出处：《石河子大学》2017年硕士论文　论文类型：学位论文

【摘要】：微藻具有摄取N、P等营养盐和有机物的功能,在与活性污泥构建共生体系后,利用两者间的相互作用将废水中的营养盐合成自身细胞物质,有效去除污水中的氮、磷和有机物,从而使废水达到净化的目的。由于生化法处理染料废水的需要,许多可降解染料的微生物被挖掘,微藻作为一种可降解染料的微生物也被广泛研究,但不同微生物其降解机制、对染料脱色效果均存在很大的差异。本论文以课题组前期筛选到的可降解分散蓝2BLN的小球藻C.sorokiniana XJK和活性污泥为研究对象,解析小球藻、活性污泥的染料脱色机理,以脱色率为指标,构建小球藻-活性污泥体系处理分散蓝2BLN染料废水,实验结果如下:(1)利用小球藻C.sorokiniana XJK处理分散蓝2BLN废水,考察培养条件对小球藻处理染料废水的影响,以脱色率为指标,获得最佳脱色条件为:染料浓度60 mg/L、接种量8?106 cell/mL、初始pH 4、光照强度470μmol/(m2·s)和CO2通气量为1%,脱色率为83%;同时获得生物量570 mg/L和油脂含量43%。通过UV-vis、FTIR、GC-MS和木质素降解酶测定的分析方法,研究小球藻降解染料分散蓝2BLN的机理,结果发现染料在560 nm处的最大吸收峰消失,发色基团断裂,并生成七种小分子物质,譬如乙苯、乙酸乙酯、丙胺等;此外,小球藻分泌地漆酶和MnP在降解分散蓝2BLN过程中发挥着重要作用,培养5天后分别获得3.89 U/mL和4.86 U/mL。(2)活性污泥对染料去除研究及其组成研究:以活性污泥为实验对象,研究活性污泥的吸附特性以及水质条件对染料脱色和污染物的去除情况,结果表明:活性污泥对染料的吸附符合假二级动力学模型和Fredundlich等温吸附模型。在投加2.5 g污泥,初始pH为8和葡萄糖浓度为0.5 g/L条件下,有利于染料脱色和去除COD等污染物。活性污泥生态系统中94.93%归属于细菌域,变形菌门(75.46%)是最丰富的门类,其次是绿弯菌(4.58%)、拟杆菌(3.4%)和浮霉菌(1.96%)。同时还检测到古菌域(0.33%)、真核域(0.24%)、绿藻门(0.01%)及未匹配到的物种。(3)小球藻-活性污泥体系构建及其对染料废水的处理优化,结果表明,藻菌体系处理废水的效率高于单藻和单污泥体系,最佳体系中小球藻和污泥的接种量浓度分别为8?106 cell/mL,污泥量为1.5 g/L。在初始pH为7、Na2SO4浓度为5 g/L和葡萄糖作为碳源的条件下,藻菌体系脱色效果最好,且对COD和TP的去除率均在90%以上。将活性污泥与小球藻置于同一体系,不仅提高了微生物的沉降性能,且对实际染料废水处理效果好,COD、氨氮和总磷的去除率分别为75%、46%和94%。以上结果说明藻菌体系有利于提高微生物处理染料废水的能力,是一种较为经济有效的生物处理方法,具有一定应用潜力。
[Abstract]:Microalgae have the function of ingesting nutrients such as NBP and organic matter. After constructing a symbiotic system with activated sludge, the nutrient salt in wastewater is synthesized into self-cellular substance by the interaction of the two. The removal of nitrogen, phosphorus and organic matter in wastewater is effective, so that the wastewater can achieve the purpose of purification. Because of the need of biochemistry to treat dye wastewater, many biodegradable microbes have been excavated. Microalgae as a biodegradable dye microorganism is also widely studied, but the degradation mechanism of different microorganisms. The effect of decolorization on dye is very different. C. sorokiniana, a biodegradable Chlorella sp. 2BLN, was selected by our research group in the early stage. XJK and activated sludge were studied. The mechanism of dye decolorization of Chlorella vulgaris and activated sludge was analyzed. Based on the decolorization rate, a Chlorella vulgaris activated sludge system was constructed for the treatment of disperse blue 2BLN dye wastewater. The experimental results were as follows: 1) using C. sorokiniana XJK to treat disperse blue 2BLN wastewater, the effects of culture conditions on the treatment of dye wastewater by Chlorella vulgaris were investigated. The optimum decolorization conditions are as follows: dye concentration 60 mg / L, inoculation amount 8? 106 cell / mL, initial pH 4, light intensity 470 渭 mol/(m2 路s) and CO2 ventilation volume was 1 and decolorization rate was 83%; At the same time, the biomass of 570 mg/L and the oil content of 43g were determined by UV-vis-FTIR GC-MS and lignin degrading enzyme. The mechanism of degradation of dye disperse blue 2BLN by Chlorella vulgaris was studied. It was found that the maximum absorption peak of dye disappeared at 560 nm, the chromophore was broken, and seven small molecular substances, such as ethylbenzene, were formed. Ethyl acetate, propylamine, etc.; In addition, the secretion of laccase and MnP by Chlorella vulgaris plays an important role in the degradation of disperse blue 2BLN. After 5 days of culture, 3.89 U / mL and 4.86 U / mL / mL activated sludge were obtained respectively. The adsorption characteristics of activated sludge and the removal of dyes and pollutants by water quality conditions were studied. The results showed that the adsorption of dyes by activated sludge was in accordance with pseudo-second-order kinetic model and Fredundlich isothermal adsorption model, and 2.5 g sludge was added. The initial pH of 8 and glucose concentration of 0.5 g / L were favorable for dye decolorization and COD removal. 94.93% in the activated sludge ecosystem belonged to the bacterial domain. (75.46) is the most abundant category, followed by Chlorophyllum (4.58). Bacteroides (3.4) and floating fungus (1.96). At the same time, it was also detected that the ancient bacteria field was 0.33 and the eukaryon area was 0.24). Construction of Chlorella microphylla activated sludge system and optimization of dye wastewater treatment. The efficiency of treatment of wastewater by algal bacteria system is higher than that of single algae and single sludge system. The inoculation concentration of Chlorella vulgaris and sludge in the optimal system is 8? When the initial pH was 7g / L and the concentration of Na _ 2SO _ 4 was 5 g / L and glucose was used as carbon source, the best decolorization effect was obtained. The removal rates of both COD and TP were above 90%. Putting the activated sludge and Chlorella vulgaris in the same system not only improved the settling performance of microorganism, but also had a good effect on the treatment of dye wastewater. The removal rates of ammonia nitrogen and total phosphorus were 75% and 94% respectively. The results showed that the algal bacteria system was beneficial to improve the ability of microbial treatment of dye wastewater and was a more economical and effective biological treatment method. It has certain application potential.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：X703;X17

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