罗红霉素短期冲击对活性污泥中氨氧化微生物丰度和多样性的影响

发布时间：2018-03-18 06:40

本文选题：罗红霉素　切入点：活性污泥　出处：《环境科学》2017年07期 　论文类型：期刊论文

【摘要】：本研究基于amo A基因,结合实时荧光定量PCR(quantitative real-time PCR,q PCR)和高通量测序技术研究罗红霉素(roxithromycin,ROX)短期冲击对活性污泥中氨氧化古菌(ammonia-oxidizing archaea,AOA)和氨氧化细菌(ammonia-oxidizing bacteria,AOB)丰度和多样性的影响.本研究共设置10种ROX浓度,不同浓度的ROX对氨氧化作用的影响差异明显,环境浓度(0.3~30μg·L~(-1))与中等浓度(300μg·L~(-1)和3000μg·L~(-1))的ROX并未对氨氧化作用产生影响;较高浓度(5 000~12 000μg·L~(-1))的ROX对氨氧化作用产生明显的抑制作用.环境浓度和中等浓度的ROX刺激了AOA增长,而较高浓度的ROX导致AOA的丰度下降.此外,除了环境中的痕量浓度(0.3μg·L~(-1)),其余浓度的ROX均导致AOB丰度下降,且下降趋势比AOA显著,说明AOA对ROX的耐受性高于AOB.高通量测序结果表明,在ROX的选择压下,AOA的OTUs多样性减少,AOB的OTUs多样性增加;但3个样品中最主要的AOA Candidatus Nitrososphaera gargensis的相对丰度随ROX浓度增加而增多,最主要的AOB Nitrosomonas eutropha的相对丰度随ROX浓度增加而减少,这同样说明了AOA对ROX的耐受性高于AOB.冗余分析结果表明:AOA Ca.Nitrososphaera gargensis、Candidatus Nitrosoarchaeum koreensis和AOB Nitrosomonas oligotropha、Nitrosomonas watsonii、Nitrosomonas halophilus均与ROX浓度呈正相关.
[Abstract]:This study is based on the amo A gene, combined with real-time fluorescence quantitative PCR (quantitative real-time PCR, Q PCR) and high-throughput sequencing technology of roxithromycin (roxithromycin, ROX) the short-term impact on the activated sludge of ammonia oxidizing archaea (ammonia-oxidizing, archaea, AOA) and ammonia oxidation bacteria (ammonia-oxidizing bacteria, AOB) of abundance and diversity. The study includes 10 kinds of concentration of ROX, effects of different concentrations of ROX on ammonia oxidation is obviously different, the environmental concentration (0.3~30 G - L~ (-1)) and medium concentration (300 g - L~ (-1) and 3000 g L~ (-1) ROX) did not affect ammonia oxidation; high concentration (5 000~12 000 G - L~ (-1)) ROX has obvious inhibitory effect on ammonia oxidation. Environment and moderate concentrations of ROX stimulated AOA growth, while higher concentration of ROX resulted in the abundance of AOA decreased. In addition, in addition to trace concentration in the environment (0.3 g L~ (-1)), the concentration of ROX resulted in AOB abundance decreased, and the decreased trend than AOA, indicating that tolerance of AOA to ROX than AOB. high-throughput sequencing results show that the selective pressure of ROX, AOA OTUs AOB OTUs to reduce the diversity, diversity increased; but the relative abundance of AOA Candidatus Nitrososphaera gargensis the main 3 samples with ROX concentration increased, the relative abundance of the main AOB Nitrosomonas eutropha decreases with the increase of ROX concentration, it also demonstrates the tolerance of AOA to ROX than AOB. redundancy analysis results show that the AOA Ca.Nitrososphaera gargensis, Candidatus Nitrosoarchaeum koreensis and AOB Nitrosomonas oligotropha, Nitrosomonas watsonii, Nitrosomonas halophilus and ROX concentration were positively correlated.

【作者单位】：北京工业大学环境与能源工程学院城镇污水深度处理与资源化利用技术国家工程实验室;
【基金】：国家自然科学基金项目(51378027,51578015) 北京市自然科学基金项目(8162010)
【分类号】：X172;X703

【相似文献】