耐盐自养铁基反硝化菌的分离鉴定及特性研究

发布时间：2018-03-18 09:12

本文选题：耐盐铁基反硝化菌　切入点：分离鉴定　出处：《高校化学工程学报》2017年02期 　论文类型：期刊论文

【摘要】：针对高盐、低C/N比废水脱氮难题,从实验室稳定运行的高效铁盐脱氮反应器中分离获得了一株耐盐自养反硝化菌株,命名为NF-5。形态观察表明,菌株NF-5个体呈杆状,大小(0.80~1.2)μm×(1.7~2.3)μm,革兰氏阳性;经生理生化试验和16S rRNA测序测定分析,菌株NF-5可归入Isoptericola属。菌株NF-5能够利用亚铁作为电子供体,利用硝酸盐作为电子受体。当起始硝氮浓度为50 mg·L~(-1)(铁氮比为5:1),菌株接种量OD_(600)为0.52(稀释18倍)时,7 d后反应体系中硝氮去除率为82%,硝氮去除速率高达0.31 mg N·(L·h)~(-1)。菌株NF-5耐盐性能良好,可耐受范围为10~50 g·NaCl·L~(-1),最适盐度为20 g·NaCl·L~(-1)。当盐浓度为50 g·NaCl·L~(-1)时,菌悬液中活细胞比例为54%,反应体系中反硝化活性仅下降47%。
[Abstract]:A salt-tolerant autotrophic denitrification strain named NF-5 was isolated from a highly efficient ferric salt denitrification reactor with high salinity and low C / N ratio. The morphological observation showed that the strain NF-5 was rod shaped. By physiological and biochemical tests and 16s rRNA sequencing analysis, the strain NF-5 can be classified into Isoptericola genus. Strain NF-5 can use ferrous as electron donor. Nitrate was used as electron acceptor. When the initial nitrate-nitrogen concentration was 50 mg 路L ~ (-1) and the inoculation amount was 0.52 (dilution 18 times), the nitrate removal rate in the reaction system was 822 after 7 days, and the nitrate removal rate was up to 0.31 mg N 路L 路h ~ (-1) ~ (-1). The salt tolerance of strain NF-5 was good. The tolerance range was 10 ~ 50 g 路NaCl 路L ~ (-1) and the optimum salinity was 20 g 路NaCl 路L ~ (-1) 路L ~ (-1). When the salt concentration was 50 g 路NaCl 路L ~ (-1), the percentage of living cells in the suspension was 54%, and the denitrification activity in the reaction system decreased only 47%.
【作者单位】：浙江大学环境工程系;
【基金】：国家自然科学基金(51278457,51578484) 国家青年基金(51608474) 浙江省创新团队(2013TD12)
【分类号】：X172

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