六溴环十二烷对斑马鱼的甲状腺激素干扰效应研究

发布时间：2018-03-18 23:04

本文选题：六溴环十二烷　切入点：斑马鱼　出处：《农业环境科学学报》2017年11期 　论文类型：期刊论文

【摘要】：作为添加型阻燃剂,六溴环十二烷(HBCDs)广泛存在于环境介质及生物体内。为研究HBCDs饲料长期暴露对斑马鱼的甲状腺激素干扰效应,设计HBCDs饲料暴露剂量为0、10、100、400 ng·g-1,对斑马鱼成鱼进行为期56 d的长期暴露,以其肝脏组织中三碘甲腺原氨酸(T3)、四碘甲腺原氨酸(T4)、游离三碘甲腺原氨酸(FT3)和游离四碘甲腺原氨酸(FT4)含量及T3/T4比值作为生物标志物,评价HBCDs饲料长期暴露对斑马鱼肝脏组织甲状腺功能的影响。结果表明,HBCDs对斑马鱼体内T3和T4具有明显抑制作用(P0.05),随着HBCDs暴露浓度的增加,T3和T4的含量水平呈现下降趋势;在中浓度组(100 ng·g-1)和高浓度组(400 ng·g-1),HBCDs对FT3和FT4具有显著性抑制作用(P0.05);随着HBCDs暴露浓度增加,T3/T4的比值呈现升高的趋势,表明HBCDs可诱导T4转化为T3。研究表明,HBCDs对鱼类具有甲状腺激素干扰效应,从而影响鱼类的生长发育。
[Abstract]:HBCDs, as additive flame retardants, are widely found in environmental media and in vivo. In order to study the thyroid hormone interference effect of HBCDs feed on zebrafish for a long time, The exposure dose of HBCDs to adult zebrafish for 56 days was designed to be 400 ng 路g ~ (-1). The contents of triiodothyronine (T3), tetraiodothyronine (T4), free triiodothyronine (FT3), free tetraiodothyronine (FT4) and the ratio of T3 / T4 were used as biomarkers. To evaluate the effect of long-term exposure of HBCDs feed on the thyroid function of liver tissue of zebrafish, the results showed that HBCDs had obvious inhibitory effect on T3 and T4 in zebrafish, and the contents of T3 and T4 decreased with the increase of HBCDs exposure concentration. The ratio of T3 / T4 to FT3 and FT4 was increased with the increase of HBCDs concentration, and the inhibitory effect of HBCDs on FT3 and FT4 was significant in the medium concentration group (100ng 路g-1) and the high concentration group (400ng 路g-1g -1), the ratio of T3 / T4 increased with the increase of HBCDs exposure concentration. The results showed that HBCDs could induce the transformation of T4 to T3.The results showed that HBCDs had thyroid hormone interference effect on fish, which affected the growth and development of fish.
【作者单位】：长安大学旱区地下水文与生态效应教育部重点实验室;环境保护部华南环境科学研究所;国家环境保护环境污染健康风险评价重点实验室;深圳市环境科学研究院;
【基金】：广东省自然科学基金(2015A030313790,2015A030313863,2014A030310002) 环境保护部华南环境科学研究所中央级公益性科研院所基本科研业务专项资助项目~~
【分类号】：X503.225

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