零价铁对污泥高温厌氧消化过程中四环素抗性基因及第一类整合子的消减影响

发布时间：2018-03-21 19:34

本文选题：零价铁　切入点：高温厌氧消化　出处：《环境科学》2017年02期 　论文类型：期刊论文

【摘要】：为研究污泥高温厌氧消化过程中零价铁(Fe0)对抗生素抗性基因的消减影响,采用荧光定量PCR方法定量检测了7种四环素抗性基因(TC-ARGs,包括tetA、tetC、tetG、tetM、tetO、tetW和tetX)及第一类整合子int I1基因在厌氧消化过程中的丰度变化,同时检测分析了挥发性脂肪酸(VFAs)的质量浓度变化,并探讨了目标基因与VFAs之间的相关关系.结果表明,适量Fe0(如0.10 g·g~(-1),以Fe0/VSS计)不仅可以强化污泥厌氧消化进程,显著提高总VFAs和乙酸的产生量,而且能够增强TCARGs和int I1基因的消减效果.尽管如此,过量Fe0(如1.17 g·g~(-1),以Fe0/VSS计)对污泥高温厌氧消化过程中TC-ARGs和int I1基因消减的增强效果并不显著,推测可能是由于基因水平转移的发生所导致的.通过相关性分析可知,TC-ARGs(除tetO基因外)和int I1基因与乙酸之间存在显著负相关性,表明乙酸可能对TC-ARGs和int I1基因的消减具有促进作用.
[Abstract]:In order to study the effect of zero-valent Fe _ (0) on the reduction of antibiotic resistance genes during high temperature anaerobic digestion of sludge, The abundance of seven tetracycline resistant genes (TC-ARGsincluding tetAtetCntetetOtetW and tetX) and the first class integron int I1 gene during anaerobic digestion were detected by fluorescence quantitative PCR method. The mass concentration of VFASs was also analyzed. The correlation between target gene and VFAs was discussed. The results showed that Fe _ 0 (0.10 g / g ~ (-1)) could not only enhance the anaerobic digestion process of sludge, but also increase the production of total VFAs and acetic acid. And it could enhance the subtractive effect of TCARGs and int I1 gene. However, the enhancement of TC-ARGs and int I1 gene subduction by excessive Fe _ 0 (1.17 g / g ~ (-1) G ~ (-1)) was not significant during high temperature anaerobic digestion of sludge. The correlation analysis showed that there was a significant negative correlation between TC-ARGs (except tetO gene) and int I1 gene and acetic acid. These results suggest that acetic acid may promote the reduction of TC-ARGs and int I 1 genes.
【作者单位】：东华大学环境科学与工程学院国家环境保护纺织工业污染防治工程技术中心;
【基金】：国家自然科学基金项目(51208086) 东华大学“励志计划”项目
【分类号】：X703.1

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