桉木APMP制浆废水抽出物提取及其抑藻效应研究

发布时间：2018-03-31 00:15

本文选题：化感物质　切入点：抑制作用　出处：《林产化学与工业》2017年02期

【摘要】：采用乙酸乙酯和甲基叔丁基醚2种有机溶剂对自制桉木碱性过氧化氢机械浆(APMP)制浆废水进行有机物提取,采用GC-MS分析对比了2种溶剂抽出物的组成。结果表明:乙酸乙酯提取的抽提物得率为204 mg/L,甲基叔丁基醚提取得率为132 mg/L,经GC-MS分析表明2种溶剂提取的物质种类分别为44种和49种,主要包含有机酸类、醇类、酯类和酚类物质,其中酸类物质的含量最高,分别为76%和80%左右。将乙酸乙酯抽提物应用于铜绿微囊藻(Microcystis aeruginosa)的抑藻效应研究,发现不同浓度的化感物质对铜绿微囊藻均具有一定程度的抑制作用,且随浓度增加抑藻效应增强。较低浓度的化感物质对铜绿微囊藻有抑制作用但不显著;高浓度的化感物质对铜绿微囊藻具有显著抑制效应,50、100 mg/L时培养7天抑制率分别达到24.8%和77.2%;藻液在446 nm的吸光值测定结果进一步证明了APMP制浆废水中提取的有机物质具有抑藻效果。
[Abstract]:Two organic solvents, ethyl acetate and methyl tert-#china_person0# ether, were used to extract organic matter from eucalyptus alkaline hydrogen peroxide pulping wastewater. The compositions of the two solvents were analyzed and compared by GC-MS.The results showed that the extraction yield of ethyl acetate was 204mg / L, the extraction rate of methyl tert-#china_person0# was 132mg / L, and the GC-MS analysis showed that there were 44 and 49 kinds of substances extracted by two solvents, mainly containing organic acids and alcohols.The contents of esters and phenols were the highest, about 76% and 80%, respectively.The inhibitory effect of ethyl acetate extract on microcystis aeruginosa was studied. It was found that allelochemicals of different concentrations could inhibit microcystis aeruginosa to a certain extent, and the inhibitory effect increased with the increase of the concentration of microcystis aeruginosa.Lower concentrations of allelochemicals inhibited microcystis aeruginosa but not significantly.High concentration of allelochemicals had a significant inhibitory effect on microcystis aeruginosa. The inhibition rates of microcystis aeruginosa cultured for 7 days reached 24.8% and 77.2% respectively at 50100 mg/L, and the absorptivity of algae solution at 446nm further proved the organic matter extracted from APMP pulping wastewater.Quality has the effect of inhibiting algae.
【作者单位】：天津科技大学造纸学院;天津市制浆造纸重点实验室;
【基金】：天津市应用基础与前沿技术研究计划重点项目(14JCZDJC40500) 天津市国家级大创项目(201610057039)
【分类号】：X793

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