典型集雨人饮地区窖水微生物群落多样性及差异解析

发布时间：2018-04-16 17:03

本文选题：典型集雨饮水地区 + 集雨窖水　；参考：《环境科学》2017年11期

【摘要】：为阐释我国典型集雨饮水地区窖水水质自然变化机制,应用16S rRNA基因-Illumina Mi Seq高通量测序技术,对以庭院为组织单元、不同类型集流面和水窖组合模式构建的集雨水源地水体中微生物群落及功能多样性差异进行了研究,表明窖水微生物群落结构及其功能存在显著的差异,造成这种差异的主要因素是窖水内部环境因子、外部环境因子等的异质性.窖水微生物群落结构多样性差异呈现大量的稀有种和少数常见优势种的分布模式,不同类型集流面和水窖组合模式构建的集雨水源地水体中含有不同的显著差异性微生物类群;微生物群落功能多样性差异体现在:编码新陈代谢功能的基因序列与其他功能基因序列相比具有显著的优势性,氨基酸、碳水化物、能量、脂类、辅助因子和维生素、萜类和酮类化合物、核苷酸、多糖的合成与代谢、外来物质的降解与代谢是构成新陈代谢功能的主要基因序列类群,表明窖水中存在大量、具有相对特定生态功能的微生物进行着诸多活跃的与新陈代谢相关活动.研究认为混凝土集流面与混凝土水窖组合模式用于收集和贮存雨水,水质更好、更安全.研究结果加深了对窖水微生物群落结构和多样性的认识,为开展集雨窖水水源地优选及改善窖水水质提供了借鉴.
[Abstract]:In order to elucidate the natural variation mechanism of cellar water quality in typical rainwater catchment areas in China, the 16s rRNA gene Illumina Mi Seq high throughput sequencing technique was used to study the structure of cellar water quality in the courtyard.The diversity of microbial communities and their functions in the water of rainwater catchments were studied by using different types of catchment surfaces and cellar combination models. The results showed that there were significant differences in the structure and function of microbial communities in pit water.The main factors causing this difference are the heterogeneity of internal environmental factors and external environmental factors.The diversity of microbial community in cellar water showed the distribution pattern of a large number of rare species and a few common dominant species.Functional diversity of microbial communities is reflected in: the sequence of genes that encode metabolic function has significant superiority over other functional gene sequences, amino acids, carbohydrates, energy, lipids, auxiliary factors and vitamins,The synthesis and metabolism of terpenoids and ketones, nucleotides and polysaccharides, and the degradation and metabolism of foreign substances are the main gene sequence groups that constitute the metabolic function.Microorganisms with relatively specific ecological functions are involved in many activities related to metabolism.It is considered that the combined model of concrete catchment surface and concrete cellar can be used to collect and store Rain Water, and the water quality is better and safer.The results of the study have deepened the understanding of the microbial community structure and diversity of the cellar water and provided a reference for developing the optimal selection of the water source of the rainwater collecting cellar water and improving the water quality of the cellar water.
【作者单位】：兰州交通大学环境与市政工程学院;城市雨水系统与水环境省部共建教育部重点实验室;甘肃农业大学资源与环境学院;
【基金】：教育部长江学者和创新团队发展计划项目(IRT_14R25);教育部城市雨水系统与水环境教育部重点实验室开放课题项目
【分类号】：X172

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