缓流水体高效脱氨氮菌株的分离及应用研究

发布时间：2018-04-29 19:44

  本文选题：缓流水体 + 脱氨氮菌　； 参考：《中原工学院》2017年硕士论文

【摘要】：针对黑臭缓流水体氨氮去除率低的问题,本研究采用改良富集培养基及现代菌株分离技术,分离培养高效脱氨氮菌株,生产制备菌剂,通过正交试验,分析菌剂氨氮去除率的影响因素,考察菌剂运用于黑臭缓流水体的效果。在其他学者研究的基础上,本研究对亚硝化菌富集用培养基进行了改进,在严格控制氨态氮含量的基础上,加入红糖,丰富碳素和微量元素。利用现代微生物分离技术,从氨氮去除率较高的污水处理厂和生活垃圾处理厂附近的污泥、污水中,分离出26株脱氮菌株,其中,16株以NH4+为氮源,分别记作X1-X16,10株以NO_2~-为氮源,分别记作菌株X17-X26。测定菌株生长速率,得到生长较快的菌株X1、X3、X4、X6、X17、X18、X19,经对菌株脱氮效率分析,筛选出氨氮去除效率最高的X1与X18混合菌株,该混合菌株的氨氮去除率可达90.0%。以红糖为主要发酵原料,将该混合菌株扩大培养,制备菌剂,并拟定菌剂的企业标准。模拟缓流水体的成分,在实验室选取氨氮初始浓度、温度、时间、pH、添加量、溶解氧6个因素,每个因素设置5个水平,通过正交试验,分析脱氨氮菌剂使用过程中的影响因素,确定最佳菌剂最佳使用条件,极差分析结果显示,影响菌剂氨氮去除率的六因素顺序为温度(29)溶解氧(29)氨氮初始浓度(29)添加量(29)时间(29)pH。确定本菌剂较佳使用条件为:温度25℃,溶解氧浓度2mg/L,氨氮初始浓度40mg/L,菌剂添加量0.3%,时间120h,pH8,此条件下本菌剂氨氮去除率最高,可达94.7%。将菌剂应用于黑臭缓流水体,得出120h后水体中氨氮去除率为82.6%和85.0%,水样嗅阈值明显减小,除臭效果明显;但与实验室脱氮效果还有一定差距,菌剂对实验室人工配水的氨氮去除率达94.7%,两者相差近13.0%。其主要原因可能为实际缓流水体的成分较为复杂,水中存在抑制微生物菌剂生长的其他微生物群落或有害物质,对脱氨氮菌剂使用效果有一定影响。因此,可以对实验室人工配水和缓流水体的性质进一步研究,不断优化菌剂的生长条件,进而提高菌剂用于缓流水体的氨氮去除率。
[Abstract]:Aiming at the problem of low removal rate of ammonia nitrogen in black-odor slow-flow water, this study adopted improved enrichment medium and modern strain isolation technology to isolate and culture high-efficiency denitrifying nitrogen strains, and to produce the preparation agent. The influence factors of ammonia-nitrogen removal rate were analyzed, and the effect of bacteria agent applied to black-odor slow-flow water was investigated. On the basis of other scholars' studies, the enrichment medium of nitrosobacteria was improved. On the basis of strictly controlling the content of ammonia nitrogen, brown sugar was added to enrich carbon and trace elements. Using modern microbial isolation technology, 26 denitrification strains were isolated from sewage treatment plants with high ammonia nitrogen removal rate and sludge near domestic waste treatment plants. Among them, 16 strains were denitrified with NH4 as nitrogen source, and 10 strains were recorded as X1-X16C- as nitrogen source. X17-X26. The growth rate of the strain X1, X3, X4, X4, X6, X17, X18, X19, was determined. The mixed strain X1 and X18, which had the highest removal efficiency of ammonia nitrogen, was screened by analyzing the denitrification efficiency of the strain. The ammonia nitrogen removal rate of the mixed strain could reach 90.0%. Using brown sugar as the main fermentation material, the mixed strain was expanded and cultured to prepare the fungicides, and the enterprise standard of the fungicides was worked out. The composition of the slow flow water was simulated, the initial concentration of ammonia nitrogen, temperature, time pH, addition amount, dissolved oxygen were selected in the laboratory, and each factor was set at 5 levels. Through orthogonal experiment, the influencing factors in the process of using ammonia nitrogen bacteria agent were analyzed. The results of range analysis showed that the six factors affecting the removal rate of ammonia-nitrogen were temperature (29) dissolved oxygen (29) initial concentration of ammonia (29) addition (29) time of 29? The optimum conditions were determined as follows: temperature 25 鈩，

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