黄顶菊种子萌发过程中DNA甲基化的MSAP分析

发布时间：2018-04-30 03:15

本文选题：黄顶菊 + 种子萌发　；参考：《植物保护学报》2017年05期

【摘要】：为明确入侵植物黄顶菊Flaveria bidentis生长发育与DNA甲基化之间的相互关系,采用甲基化敏感扩增多态性(methylation sensitive amplification polymorphism,MSAP)方法研究了黄顶菊种子萌发过程中DNA甲基化的动态变化。结果表明,DNA甲基化与去甲基化两者共同调控黄顶菊生命初期的生长发育,且去甲基化的变化在种子萌发过程中占主导。筛选出的12对引物共扩增出998条MSAP条带,其中多态性条带为951条,多态性百分比为95.37%。黄顶菊种子萌发过程中胞嘧啶发生甲基化主要以双链甲基化形式为主,位点数为94个,而单链甲基化位点数仅为50个;多态性位点数占总位点数比率为48.95%,表明有近一半的位点发生了DNA甲基化和去甲基化的变化;发生去甲基化变化的多态性片段有73个,而发生甲基化变化的有21个,说明黄顶菊种子萌发阶段DNA甲基化的变化主要以去甲基化形式为主,且在萌发第4天后去甲基化数目持续快速上升。
[Abstract]:In order to clarify the relationship between Flaveria bidentis growth and DNA methylation in invasive plant, the dynamic changes of DNA methylation during seed germination were studied by methylation sensitive amplified polymorphic sensitive amplification polymorphism (MSAP) method. The results showed that both DNA methylation and demethylation regulated the growth and development of Chrysanthemum chinensis in the early stage of life, and the change of demethylation played a dominant role in the process of seed germination. A total of 998 MSAP bands were amplified from 12 pairs of primers, including 951 polymorphic bands, and the percentage of polymorphic bands was 95.3737. During seed germination, cytosine methylation mainly occurred in the form of double strand methylation, with 94 site points and 50 single strand methylation sites. The percentage of polymorphic sites to total sites was 48.95, indicating that nearly half of the sites had DNA methylation and demethylation, and 73 polymorphic fragments had demethylation changes, while 21 had methylation changes. The results showed that the change of DNA methylation was mainly in the form of demethylation during germination, and the number of demethylation increased rapidly after 4 days of germination.
【作者单位】：农业部环境保护科研监测所;山西农业大学;天津农学院;
【基金】：国家自然科学基金(31401811) 天津市应用基础与前沿技术研究计划(15JCQNJC15300)
【分类号】：Q16

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