萌发期硫酸铜胁迫对绿豆生长的影响及其细胞内密度变化反应

发布时间：2018-05-06 04:26

本文选题：绿豆 + 硫酸铜　；参考：《植物生理学报》2016年12期

【摘要】：绿豆(Vigna radiata)在硫酸铜胁迫下水培发芽后再移植土种,观察胚芽和幼苗生长情况;测定胚芽和幼苗细胞内密度变化(DANCE反应),以及胚芽的超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,研究萌发期硫酸铜胁迫对绿豆生长的影响及其DANCE反应。结果显示,硫酸铜胁迫抑制胚芽生长,以胁迫3~4 d最为显著,并出现芽细胞DANCE反应。移植土壤后,胁迫组幼苗出现代偿性加快生长,生长率随萌发期硫酸铜胁迫浓度增加而提高。土种3 d后,胁迫组幼苗表观生长状态恢复正常,但是,胁迫组幼苗根、茎、叶均出现DANCE反应。根、茎、叶部在土种1~2 d后均发生DANCE反应,其中根部反应持续1 d,茎部反应持续3 d,而叶部反应则持续至土种第5日。土种6 d后,各胁迫组幼苗均无DANCE反应。硫酸铜胁迫还导致胚芽SOD活性和MDA含量显著增高,SOD基因m RNA表达增加,提示硫酸铜胁迫促进胚芽产生自由基。研究结果表明,绿豆的DANCE反应可能是发芽减慢和幼苗代偿性生长所致,发芽期适当的环境胁迫可能有助于植物幼苗生长。
[Abstract]:Vigna radiata was transplanted into soil after incubation and germination under copper sulfate stress, the growth of embryo and seedling was observed, the changes of intracellular density of germ and seedling were determined, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDAA) in germ were determined. The effects of copper sulfate stress during germination on the growth of mung bean and its DANCE response were studied. The results showed that cupric sulfate stress inhibited the growth of germ, and the DANCE reaction of bud cells appeared in the stress of 3 ~ 4 days. After transplanting soil, the seedlings of stress group developed modern compensatory growth, and the growth rate increased with the increase of copper sulfate stress concentration in germination period. After 3 days of soil planting, the apparent growth state of the seedlings in the stress group returned to normal, but DANCE reaction appeared in the roots, stems and leaves of the seedlings in the stress group. DANCE reaction occurred in root, stem and leaf after 1 ~ 2 days. The root reaction lasted 1 day, the stem reaction lasted 3 days, and the leaf reaction lasted until the 5th day. After 6 days, no DANCE response was found in the seedlings of each stress group. Copper sulfate stress also increased the activity of SOD and the content of MDA in germs, suggesting that copper sulfate stress could promote the production of free radicals. The results showed that the DANCE reaction of mung bean might be caused by slow germination and compensatory growth of seedlings, and proper environmental stress at germination stage might be helpful to plant seedling growth.
【作者单位】：暨南大学医学院生化系;广州市东来生物科技有限公司;
【基金】：中国科技部科技创新基金(12C26214405312)~~
【分类号】：S522

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