4种农药对青海弧菌Q67混合毒性作用的评估

发布时间：2018-05-31 01:42

本文选题：农药 + 四元混合物　；参考：《安徽农业大学学报》2017年05期

【摘要】：以发光菌青海弧菌(Vibrio qinghaiense sp.-Q67)为指示生物,4种农药包括残杀威、甲霜灵、多果定和西草净为研究对象,应用均匀试验设计法设计农药的四元混合物体系,共7条射线,应用微板毒性测试方法(MTA)系统测定4种农药及其四元混合物体系对Q67的发光抑制毒性,采用非线性最小二乘法拟合浓度-效应数据,以浓度加和模型(CA)为标准加和参考模型分析混合物毒性相互作用。结果表明,Logit或Weibull函数可有效地拟合4种农药的浓度效应数据,其相关系数R0.99,均方根误差RMSE0.022;4种农药对发光菌Q67的毒性大小顺序为多果定(p EC50=5.35)西草净(p EC50=3.56)甲霜灵(p EC50=3.18)残杀威(p EC50=3.00),其中多果定毒性最大,残杀威、甲霜灵和西草净对Q67的毒性比较接近;四元农药混合物体系的7条射线对Q67的毒性(p EC50值)与组分西草净和多果定的浓度比尤其是西草净和多果定的浓度比之和具有良好的正相关关系(R=0.8729,RMSE=0.076),但与残杀威和甲霜灵的浓度比之和具有良好的负相关关系(R=0.8729,RMSE=0.076);CA模型能很好地评估四种农药的四元混合物体系对Q67的毒性,即混合物体系呈经典的加和作用。
[Abstract]:Vibrio qinghaiense sp.-Q67) was used as indicator for four kinds of pesticides, including propoxur, Metalaxyl, Duoguodin and Xicaojing, and the four-component mixture system of pesticides was designed by uniform experimental design method, which was composed of 7 rays, and was used for the study of the chemical composition of Vibrio qinghaiense sp.-Q67 (Vibrio qinghaiense sp.-Q67). The luminescence inhibition toxicity of four pesticides and their quaternary mixtures to Q67 was determined by using the microplate toxicity test method (MTAA). The concentration effect data were fitted by nonlinear least square method. The toxicity interaction of mixtures was analyzed by using the concentration sum model (CAA) as the standard additive model and the reference model. The results showed that the logit or Weibull function could fit the concentration effect data of four pesticides effectively. The correlation coefficient R0.99, root mean square error (RMSE 0.022), the toxicity order of four pesticides to the luminescent bacteria Q67 were as follows: the order of toxicity of the four pesticides to the luminescent bacteria Q67 was: the toxicity of the four pesticides was polyguodine (EC50 / 5. 35) Xicaojing (pEC5050 / 3.56) Metalaxelin (pEC50EC50 / 3.18) propoxapine (EC503.00), among which the toxicity of Doguodine was the greatest and propoxur was the most important. The toxicity of Metalaxyl and Xicaojing to Q67 was similar. The toxicity of seven rays to Q67 (EC50 value) has a good positive correlation with the concentration ratio of Xichaojing and Doguodine, especially the concentration ratio of Xicaojing to Doguodine, but it has a good positive correlation with propoxur and Doguodine, but it has a good positive correlation with the sum of the concentrations of Xicaojing and Doguodine. There was a good negative correlation between the concentration ratio and the concentration ratio of methylaniline. The model of RX 0.8729 (RMSE) 0.076C can be used to evaluate the toxicity of four pesticide quaternary mixtures to Q67. That is, the mixture system has a classical additive effect.
【作者单位】：安徽建筑大学环境与能源工程学院安徽省水污染控制与废水资源化重点实验室;清华大学新兴有机污染物控制北京市重点实验室;
【基金】：国家自然科学基金(21677001,21207002) 安徽省自然科学基金(1708085MB50) 安徽省高等学校自然科学研究项目(KJ2013A067)共同资助
【分类号】：X171.5

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