白腐菌的固定化及其对酸性黑ATT的脱色研究

发布时间：2018-06-04 21:24

本文选题：黄孢原毛平革菌 + 吸附固定化　；参考：《天津科技大学》2017年硕士论文

【摘要】：酸性黑ATT是典型的复合型偶氮染料,具有可生化性差、色度高、成分复杂、直接排放于环境中会造成严重污染等特点,脱色处理成为处理该类废水的关键环节。目前对于该类染料污染的废水处理鲜有报道。本文将固定化技术与白腐菌生物处理相结合,利用吸附固定化白腐菌、包埋固定化白腐菌分别对酸性黑ATT进行脱色处理。课题首先采用气相色谱-质谱联用(GC-MS)对脱色后的酸性黑ATT溶液中的物质进行检测,对降解后可能存在的物质进行分析,对比前后物质结构的变化,证明了白腐菌对酸性黑ATT的脱色作用不仅仅是微生物的生物吸附,还有发色N=N结构的断裂的原因。在吸附固定化实验中,将游离态白腐菌分别吸附固定化在葵花盘、葵花秸秆、葵花籽皮、玉米芯、花生壳等经过改性后的植物材料上,投入到酸性黑ATT模拟染料废水中进行脱色。对比了各空白载体的机械强度、吸附、解吸以及菌体附着情况,分别考察了不同载体、温度、摇床转速、染料废水初始pH、染料废水浓度等因素对酸性黑ATT脱色效果的影响。结果表明:用葵花盘和葵花秸秆固定化白腐菌对酸性黑ATT的脱色效果较好。葵花盘对酸性黑ATT脱色的最适条件为pH 5.0、温度30℃、转速150rpm、染料初始浓度100ppm,葵花秸秆的最适条件为pH 5.0、温度22℃、转速150rpm、染料初始浓度100ppm,两载体在最佳条件下25h脱色率分别可达94.41%、90.49%。最佳条件下连续脱色5次,两种载体对酸性黑ATT的脱色率仍能保持在87%以上。在包埋固定化实验中,本文利用海藻酸钠(SA)、聚乙烯醇(PVA)、活性炭共同对白腐菌进行固定化。以固定化小球的成型效果、硬度以及弹性为依据,对包埋固定化材料海藻酸钠(SA)和聚乙烯醇(PVA)配比进行调整,进而考察了不同固化时间、培养时间对酸性黑ATT废水脱色效果的影响。结果表明:包埋固定化配比为1%:7%时制得的包埋固定化小球性能最好;确定固化时间和培养时间分别为90 h和5d,此时包埋固定化小球对酸性黑ATT的脱色率可达到78.13%。实验对包埋固定化小球的机械强度、热稳定性、膨胀率、比表面积、孔径分布、孔容以及传质等性能进行检测,结果表明:固定化小球强度系数可达到93%,耐受温度可达85℃;固定化小球的膨胀率1.459;比表面积27.73 m2/g,孔容0.0533 cm3/g,平均孔径19.11 nm;采用此种包埋固定化方法下的固定化小球的传质性能为99.46%,扫描电子显微镜显示固定化小球具有致密的孔隙。在采用包埋固定化白腐菌对酸性黑ATT进行脱色过程中,本文分别考察了不同载体、温度、摇床转速、染料废水初始pH、染料废水浓度等因素对酸性黑ATT脱色率的影响。结果表明:包埋固定化白腐菌对酸性黑ATT的脱色,其适宜条件为pH3.5,温度30℃,转速150 rpm时,染料初始浓度150 ppm,该条件下对酸性黑ATT连续脱色次数可达8次,最大脱色率均保持在81%左右,为固定化白腐菌对染料废水脱色的研究提供了理论依据。本文通过大量实验证实了固定化白腐菌对复合偶氮染料废水具有很好的脱色效果,对于实际废水处理具有较好的应用前景。
[Abstract]:Acid black ATT is a typical compound azo dye, which has the characteristics of poor biodegradability, high chromaticity and complex composition, which will cause serious pollution in the environment. Decolorization treatment becomes the key link in treating this kind of wastewater. At present, there are few reports on the treatment of wastewater contaminated by this kind of dye. The acid black ATT was Decolorated by adsorption and immobilization of white rot fungi and immobilized white rot fungi. First, gas chromatography-mass spectrometry (GC-MS) was used to detect the substances in the acid black ATT solution after decolorization. The substances that may exist after degradation were analyzed, and the changes of material structure before and after were compared. It is proved that the decolorization of white rot fungus to acid black ATT is not only the biological adsorption of microorganisms, but also the cause of the fracture of the hair color N=N structure. In the adsorption immobilization experiment, the free white rot fungus is adsorbed on the sunflower plate, sunflower stalk, sunflower seed skin, corn cob, peanut shell and so on, and put into the modified plant material. The decolorization of acid black ATT simulated dye wastewater was carried out. The mechanical strength, adsorption, desorption and adhesion of the blank carriers were compared. The effects of different carriers, temperature, rocking speed, initial pH of dye wastewater, dye wastewater concentration and other factors on the decolorization effect of acid black ATT were investigated. The decolorization effect of immobilized white rot fungi on acid black ATT is better. The optimum conditions for the decolorization of acid black ATT are pH 5, temperature 30, speed 150rpm, initial dye concentration 100ppm, the optimum conditions for sunflower straw are pH 5, temperature 22, 150rpm, initial dye concentration 100ppm, and two carrier in the optimum condition, the 25h decolorization rate can reach 9, respectively. 4.41%, 4.41% continuous decolorization under the best condition, and the decolorization rate of acid black ATT can remain above 87% by two kinds of carrier. In the immobilization experiment, this paper uses sodium alginate (SA), polyvinyl alcohol (PVA) and activated carbon to immobilize the white rot fungi. The results are based on the molding effect, hardness and elasticity of the immobilized ball. The ratio of sodium alginate (SA) and polyvinyl alcohol (PVA) was adjusted, and the effect of different curing time and incubation time on the decolorization effect of acid black ATT wastewater was investigated. The results showed that the immobilized and immobilized small sphericity of 1%: 7% was the best, and the curing time and incubation time were 90 h and 5D, respectively. At this time, the decolorization rate of embedded immobilized pellets to acid black ATT can be achieved by testing the mechanical strength, thermal stability, expansion rate, specific surface area, pore size distribution, Kong Rong and mass transfer properties of the embedded immobilized pellets by 78.13%. test. The results show that the strength coefficient of the immobilized ball can reach 93%, the tolerance temperature is up to 85 degrees C, and the immobilized ball is fixed. The expansion rate is 1.459, the specific surface area is 27.73 m2/g, the pore volume is 0.0533 cm3/g and the average pore size is 19.11 nm. The mass transfer performance of the immobilized pellets under this embedding fixation method is 99.46%, and the scanning electron microscope shows that the immobilized pellets have dense pores. The effects of different carriers, temperature, rocking speed, initial pH of dye wastewater, dye wastewater concentration and other factors on the decolorization rate of acid black ATT were investigated. The results showed that the suitable conditions for decolorization of acid black ATT by embedding immobilized white rot fungi were pH3.5, temperature 30 C and speed 150 rpm, the initial dye concentration was 150 ppm, and under this condition acid black A The number of continuous decolorization of TT can reach 8 times and the maximum decolorization rate remains about 81%. It provides a theoretical basis for the study of decolorization of dye wastewater by immobilized white rot fungi. This paper has proved that the immobilized white rot fungi have good decolorization effect on the compound azo dye wastewater through a large number of experiments, and has a good application prospect for the treatment of practical wastewater.
【学位授予单位】：天津科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：X703;X172

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