大气细颗粒物亚慢性染毒对大鼠肺内质网应激相关因子表达的影响

发布时间：2018-06-13 12:51

本文选题：PM. + 大鼠　；参考：《环境科学学报》2017年11期

【摘要】：采集冬、夏季太原市大气细颗粒物(PM2.5),并制备PM2.5生理盐水混悬液.将35只雄性SD大鼠随机分为7组:对照组、3个不同剂量夏季PM2.5染毒组(0.2、0.6、1.5 mg·kg~(-1)体重)及3个不同剂量冬季PM2.5染毒组(0.3、1.5、2.7 mg·kg~(-1)体重),每组5只,气道滴注法染毒,每隔2 d染毒1次,共60 d.采用荧光实时定量PCR、Western blot、ELISA方法检测大鼠肺内质网应激指标——葡萄糖调节蛋白78(GRP78)、活化转录因子6(ATF6)、C/EBP同源蛋白(CHOP)、半胱氨酸天冬氨酸蛋白酶12(Caspase12)及血红素氧合酶-1(HO-1)的mRNA和蛋白表达变化.结果表明,与对照组相比,冬季中、高剂量PM2.5染毒组大鼠肺组织GRP78、ATF6、CHOP、Caspase12、HO-1 mRNA和蛋白表达显著增加,夏季高剂量PM2.5染毒组大鼠肺组织这5个基因mRNA和蛋白表达显著增加.冬季和夏季PM2.5组大鼠肺上述5个基因表达有剂量-效应关系.结果表明,太原市PM2.5亚慢性染毒可诱导大鼠肺内质网应激相关基因GRP78/ATF6/CHOP/HO-1表达,说明肺内质网应激反应加强;而CHOP和Caspase-12上调,提示与细胞凋亡关联的内质网相关性死亡途径被激活.冬季和夏季PM2.5引起内质网应激相关因子表达上调的效应没有显著差别.
[Abstract]:PM2.5 was collected from atmospheric fine particles in Taiyuan in winter and summer, and PM2.5 saline suspension was prepared. 35 male Sprague-Dawley rats were randomly divided into 7 groups: control group, 3 different doses of PM2.5 exposure group in summer (0.2g / kg 路kg ~ (-1) and 3 different doses of winter PM _ (2.5) exposure group (n = 5) and 3 different doses of winter PM _ (2.5) exposure group (n = 5). Each group (n = 5) was administrated by airway instillation once every 2 days. A total of 60 days. The changes of mRNA and protein expression of rat lung endoplasmic reticulum stress index, Glucose-regulated protein 78GRP 78, activated transcription factor 6 ATF6, CpEBP homologue protein CHOPOP, cysteine aspartate proteinase 12 (Caspase12) and heme oxygenase-1HO-1 (HO-1) were detected by real-time quantitative PCR- Western blottmeter Elisa. The results showed that the mRNA and protein expression of GRP78 / ATF6 / CHOP1 Caspase12HHO-1 in lung tissue of rats exposed to high dose PM2.5 in winter was significantly higher than that of control group, and the mRNA and protein expression of these 5 genes in lung tissue of rats exposed to high dose PM2.5 in summer was significantly higher than that of control group. The expression of these five genes in the lung of PM2.5 group in winter and summer was dose-effect. The results showed that PM2.5 subchronic exposure could induce the expression of GRP78 / ATF6 / CHOP-HO-1 in rat lung endoplasmic reticulum stress, indicating that the stress response of lung endoplasmic reticulum was enhanced, while chop and Caspase-12 were up-regulated, suggesting that the endoplasmic reticulum associated death pathway associated with apoptosis was activated. There was no significant difference between winter and summer in the upregulation of endoplasmic reticulum stress-related factors induced by PM2.5.
【作者单位】：山西大学环境科学研究所;山西大学生物技术研究所;
【基金】：国家自然科学基金(No.91543202)~~
【分类号】：R114;X51

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