抗生素抗性基因在养殖废水中的分布与去除

发布时间：2018-06-16 19:14

本文选题：微生物固化曝气技术 + 高通量荧光定量PCR　；参考：《环境化学》2017年11期

【摘要】：畜禽养殖场作为抗生素抗性基因的一个热点区,其产生的废水中存在大量的抗生素抗性基因,直接排放将会污染接受的水体.本研究以微生物固化曝气技术为核心,构建了一个包含沉淀池、一级处理池、二级处理池和氧化塘的养殖废水处理系统.采用高通量荧光定量PCR技术,探究各种抗性基因在进水、各处理池和出水中的种类、多样性和丰度的变化以及可能的影响因素.研究发现:(1)微生物固化曝气技术能有效降低养殖废水中抗性基因的种类和多样性;(2)该技术也能有效去除养殖废水中抗性基因的绝对丰度,去除率高达93.6%;(3)养殖废水出水中抗性基因绝对丰度仍高于自然水体,其直接排放仍有抗性基因污染风险;(4)畜禽养殖废水中TN和土霉素的含量与许多抗生素抗性基因总丰度存在正相关性.通过对TN和土霉素的去除或者控制使用,可以有效降低养殖废水中的抗性基因.
[Abstract]:As a hot spot of antibiotic resistance genes, livestock farms produce a large number of antibiotic resistance genes in their wastewater, which will pollute the accepted water body directly. Based on microbial solidified aeration technology, a system of aquiculture wastewater treatment including sedimentation tank, primary treatment tank, secondary treatment tank and oxidation pond was constructed. High throughput fluorescence quantitative PCR technique was used to investigate the variety, diversity and abundance of various resistance genes in the influent, in each treatment tank and in the effluent, as well as the possible influencing factors. It is found that the microorganism solidified aeration technology can effectively reduce the species and diversity of resistance genes in aquaculture wastewater. This technique can also effectively remove the absolute abundance of resistance genes in aquaculture wastewater. The absolute abundance of resistance gene in the effluent of culture wastewater is still higher than that in the natural water. The content of TN and oxytetracycline in livestock and poultry wastewater was positively correlated with the total abundance of many antibiotic resistance genes. Removal or control of TN and oxytetracycline can effectively reduce resistance genes in culture wastewater.
【作者单位】：上海应用技术大学生态技术与工程学院;中国科学院城市环境研究所;
【基金】：上海应用技术大学引进人才科研启动项目(YJ2015-1)资助~~
【分类号】：X713

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