养殖水体邻苯二甲酸二乙酯暴露对凡纳滨对虾壳膜几丁质酶的影响

发布时间：2018-06-20 12:07

本文选题：邻苯二甲酸二乙酯 + 凡纳滨对虾(Litopenaeus　；参考：《淡水渔业》2017年06期

【摘要】：邻苯二甲酸二乙酯(DEP)是常用的塑化剂,也是环境广泛存在的内分泌干扰物。采用酶促反应动力学法及实时荧光定量PCR检测分析养殖水体DEP暴露对凡纳滨对虾(Litopenaeus vannamei)壳膜几丁质酶的比活力和酶系列基因mRNA表达量的影响。结果显示不同浓度组的DEP暴露对壳膜几丁质酶比活力的影响不具有显著性;10μg/L DEP暴露120 h后,对虾壳膜几丁质酶系的LV-CHT2、LV-CHT3、LV-CHT7等三个基因的mRNA表达量均被显著抑制,但随着暴露时间延长,这种显著性差异转化成非显著性;而20μg/L DEP胁迫对LVCHT2、LV-CHT3、LV-CHT7等三个基因的mRNA表达量没有显著性影响,不同浓度组的DEP暴露不同时间后对LV-CHT5、LV-CHT6两个基因的mRNA表达量也均不具有显著性影响。
[Abstract]:Diethyl phthalate (DEP) is a common plasticizer and endocrine disruptor. The effects of DEP exposure on chitinase activity and mRNA expression of chitinase of Litopenaeus vannamei) in cultured water were detected by enzymatic reaction kinetics and real-time quantitative PCR. The results showed that the specific activity of chitinase in shell membrane was not significantly affected by DEP exposure at different concentrations. After 120 hours of exposure to 10 渭 g / L DEP, the mRNA expression of LV-CHT2, LV-CHT3, LV-CHT7 and LV-CHT7 were significantly inhibited in shrimp shell membrane chitinase system. However, with the prolongation of the exposure time, the significant difference was transformed into non-significant difference, while 20 渭 g / L DEP stress had no significant effect on the mRNA expression of LVCHT2, LV-CHT3, LV-CHT7, etc. The mRNA expression of LV-CHT5 and LV-CHT6 genes were not significantly affected by DEP exposure at different concentrations.
【作者单位】：泉州师范学院化工与材料学院;惠安县净峰镇政府;
【基金】：国家自然科学青年基金项目(31302190) 福建省自然科学基金项目(2012J01047) 泉州市优秀人才培养专项(16D04)
【分类号】：X503.225;X714

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