氧化石墨烯对双壳类动物文蛤的亚致死毒性研究

发布时间：2018-06-25 15:51

本文选题：氧化石墨烯(GO) + 双壳类　；参考：《中国环境科学》2017年07期

【摘要】：为全面评价纳米材料对海洋生物的毒性效应,将文蛤暴露于含氧化石墨烯(GO,5mg/L)的人工海水,测定其内脏中的还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)、丙二醛(MDA)、乙酰胆碱酯酶(AChE)、金属硫蛋白(MTs)以及血细胞的微核率(MNF)和溶酶体膜稳定性(LMS)等7种生物标志物的变化,分析GO对双壳类的亚致死毒性机制.结果表明,GO能够诱导文蛤内脏的氧化逆境,表现为GSH含量降低、GSSG含量增加和MDA含量增加;GO产生的神经毒性较小,AChE活性仅在暴露初期与结束时短暂受抑;GO对MTs无诱导作用;GO暴露4d后即产生显著的遗传毒性和溶酶体膜失稳,MNF达到对照组的6.1~9.0倍,中性红保留时间(NRRT)降幅达到24.2%~49.2%.除AChE、MTs外,其它生化指标均适于作为指示GO亚致死毒性的敏感生物标志物.
[Abstract]:In order to evaluate the toxicity of nanomaterials to marine organisms, Meretrix Meretrix was exposed to artificial seawater containing graphene oxide (GOA 5 mg / L). The changes of reduced glutathione (GSH), oxidative glutathione (GSSG), malondialdehyde (MDA), acetylcholinesterase (ache), metallothionein (MTs), micronucleus rate (MNF) and lysosomal membrane stability (LMS) in the viscera were measured. To analyze the sublethal toxicity mechanism of go to bivalves. The results showed that go could induce oxidative stress in the viscera of Meretrix Meretrix. It was shown that GSH content decreased, GSSG content increased and MDA content increased. The neurotoxicity of go was less. The activity of ache was only temporarily inhibited by go at the beginning and end of exposure. After 4 days of exposure, go had significant genotoxicity. The MNF of lysosomal membrane was 9.0 times higher than that of the control group. Neutral red retention time (NRRT) decreased to 24.2kyong 49.2. With the exception of AChEMA MTs, the other biochemical indexes were suitable as sensitive biomarkers for indicating the sublethal toxicity of go.
【作者单位】：中国海洋大学海洋环境与生态教育部重点实验室;
【基金】：国家自然科学基金资助项目(41276104)
【分类号】：X171.5

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