RBER生物降解溴酸盐及微生物群落结构分析

发布时间：2018-07-08 11:58

  本文选题：溴酸盐 + 硝酸盐　； 参考：《中国环境科学》2017年05期

【摘要】：溴酸盐(BrO_3~-)是由于含溴离子(Br~-)的水体经过臭氧或加氯消毒后产生的消毒副产物,在毒理致癌性分级上被国际癌症研究机构定为II类致癌物.实验设计了一种旋转电极生物膜反应器系统(RBER),可将浓度范围为150~800μg/L的溴酸盐降解,且溴酸盐被完全还原为溴离子,没有其他中间产物生成.在反应器运行过程中发现,硝酸盐与溴酸盐对氢电子供体存在明显的竞争关系,且硝酸盐对氢电子供体竞争能力强于溴酸盐.选取反应器运行不同阶段的生物膜样品进行DNA提取和高通量测序分析微生物群落多样性.结果表明,优势菌属主要有Bacillus(枯草杆菌属)、Pseudomonas(假单胞菌属)和Lactococcus(乳球菌属)3类,其在总菌属中相对丰度占比分别为37.0%、16.2%和11.1%,可能是实现溴酸盐生物降解的主要优势菌群.
[Abstract]:Bromate (BrO3-) is a by-product of disinfection caused by ozone or chlorination in bromo-containing water. It is classified as a class II carcinogen by the International Agency for Cancer Research (IARC) in toxicological carcinogenicity classification. A rotating electrode biofilm reactor system (RBER) was designed to degrade bromate in the concentration range of 150 渭 g / L to 800 渭 g / L, and the bromate was completely reduced to bromine ion without any other intermediate products. During the operation of the reactor, it was found that nitrate and bromate had obvious competitive relationship with hydrogen electron donor, and the competition ability of nitrate to hydrogen electron donor was stronger than that of bromate. Biofilm samples at different stages of operation were selected for DNA extraction and high throughput sequencing to analyze the diversity of microbial communities. The results showed that the dominant genera were Bacillus Pseudomonas (Pseudomonas) and Lactococcus (Lactococcus). The relative abundance of Bacillus and Lactococcus were 37.0% and 11.1%, respectively, which might be the dominant bacteria for the biodegradation of bromate.
【作者单位】： 湖南省环境保护科学研究院;水污染控制技术湖南省重点实验室;湖南大学环境科学与工程学院;
【基金】：国家自然科学基金资助项目(51378188,51478170) 国家国际科技合作专项项目(2013DFG91190)
【分类号】：X172
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本文编号：2107355