1株异养硝化-好氧反硝化细菌DK1的分离鉴定及其脱氮特性

发布时间：2018-07-17 02:52

【摘要】：从某反应器活性污泥中分离筛选出1株假单胞菌属(Pseudomonas sp.)细菌,命名为DK1,并对该菌进行脱氮特性研究.在以葡萄糖为碳源,C/N量比为5时,分别以NaNO_3和NaNO_2为氮源,二者的好氧反硝化速率为4.09 mg·(L·h)-1和4.43mg·(L·h)~(-1).以二者同时为氮源脱氮率为100%;此外,菌株DK1具有异养硝化性能,NH_4~+-N平均去除速率为2.32mg·(L·h)-1.缺氧时以NO_2~--N为氮源菌株DK1可将一系列梯度浓度NO_2~--N(约100~300 mg·L-1)在36 h内降为0.当NO_3~--N和NO_2~--N同时存在时,菌株DK1会优先利用NO_3~--N进行反硝化.同时该菌株还具有同步硝化反硝化(SND)性能,可同时去除NH_4~+-N、NO_2~--N或NH_4~+-N、NO_3~--N,30 h内脱氮率分别达95.06%和94.69%.相同时间内在NH_4~+-N、NO_2~--N和NO_3~--N三者均存在时,脱氮效果最佳,达100%.菌株DK1的高效SND及反硝化性能表明其在处理含氮废水方面有一定的潜力和应用价值.
[Abstract]:A strain of Pseudomonas sp. was isolated from activated sludge of a reactor. Bacteria, named DK1, were studied for denitrification. When the C / N ratio of glucose was 5, the aerobic denitrification rates were 4.09 mg (L h) -1 and 4.09 mg (L h) -1 and 4.43mg (L h) ~ (-1), respectively, using nano _ 3 and nano _ 2 as nitrogen sources. The nitrogen removal rate of strain DK1 was 100 and the average removal rate of NH4 ~ -N was 2.32mg (Lh) -1 with heterotrophic nitrification of strain DK1. Under hypoxia, a series of gradient concentrations of no _ 2N (about 100 ~ 300 mg 路L ~ (-1) could be reduced to 0 in 36 h by using no _ 2 N as nitrogen source strain DK1. Strain DK1 will give priority to denitrification by using NO3 + -N in the presence of NO3 + -N and NO2 + -N at the same time. At the same time, the strain also has the property of simultaneous nitrification and denitrification (SND), and can simultaneously remove NH4- NnnO2-N or NH4- N- N- N- No3- N in 30 h, and the denitrification rates are 95.06% and 94.69%, respectively, within 30 h. The denitrification rates of this strain are 95.06% and 94.69% respectively. In the same time, the denitrification effect is the best, up to 100%, when NH4- N- NnnO2-N and NO3 N are all present. The high efficiency SND and denitrification performance of strain DK1 showed that DK1 had a certain potential and application value in the treatment of nitrogen-containing wastewater.
【作者单位】：北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室北京市污水脱氮除磷处理与过程控制工程技术研究中心;新加坡国立大学土木与环境工程学院;
【基金】：国家重点研发计划项目(2016YFC0401103) 高等学校学科创新引智计划项目(005000522106) 北京市教委资助项目
【分类号】：X172;X703

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