生物膜中EPS与微生物的分布及测定

发布时间：2018-07-25 14:01

【摘要】：生物膜作为微生物的载体,其组成、结构对生物膜反应器的工作性能具有重要影响。以城市污水处理厂和实验室混合处理系统两种典型的生物膜为对象,利用荧光染色和FISH技术研究生物膜中EPS与微生物的分布特征,同时考察不同提取方法对生物膜中EPS含量的影响。结果表明,两种生物膜均呈现典型的非均相体系,内部存在非均一的空洞和孔隙;EPS呈网状结构,构成生物膜的骨架,微生物填充其中;生物膜表层由于基质充足,微生物数量较高,而在生物膜内部,由于基质扩散限制和内源呼吸作用,微生物数量较低;生物膜表层主要为异养菌,硝化菌主要生长在内层;EPS中蛋白质的含量沿生物膜深度方向逐渐减少,而多糖含量则与之相反。相比于实际生物膜,实验室生物膜厚度较大,表面粗糙,VSS/SS值较高,耗氧速率也较大。各提取方法提取的EPS含量由多到少依次为:甲醛+Na OH加热CER。
[Abstract]:The composition and structure of biofilm as the carrier of microorganism have important influence on the performance of biofilm reactor. Two typical biofilms from municipal wastewater treatment plant and laboratory were used to study the distribution of EPS and microorganism in biofilm by fluorescence staining and FISH technique. At the same time, the effects of different extraction methods on EPS content in biofilm were investigated. The results showed that both biofilms presented typical heterogeneous systems, and there were heterogeneous voids and pores in EPS, which formed the skeleton of biofilm and was filled with microorganisms, and the surface layer of biofilm was filled with sufficient matrix. In the biofilm, the number of microorganism is lower because of the restriction of matrix diffusion and endogenous respiration, and the surface layer of biofilm is mainly heterotrophic bacteria. The protein content of nitrifying bacteria in the inner layer of EPS decreased gradually along the depth of the biofilm, but the content of polysaccharide was opposite. Compared with the actual biofilm, the biofilm is thicker in laboratory, higher in VSS / SS value and higher in oxygen consumption rate. The content of EPS extracted by each extraction method was as follows: formaldehyde NaOH heated CERs.
【作者单位】：西安建筑科技大学环境与市政工程学院;
【基金】：国家自然科学基金资助项目(50878178)
【分类号】：X703;X835

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