一种酶抗逆助剂的制备及其在废水净化处理中应用
发布时间：2018-08-12 15:58
【摘要】：近海岸海水、船舶废水(压载水、洗舱水、脱硫废液)等污废水中均含有较高浓度的氨氮和石油烷烃。对这类废水的生物净化处理,要求具有高盐适应性、能同时降解转化氨氮和石油烷烃的特性及高效性。本文拟利用抗逆助剂协助下的复合酶制剂污废水净化处理技术,进行模拟船舶废水脱氮和石油烷烃降解研究。本文从大连某盐场的盐池底泥中分离筛选出一株能够合成Ectoine的菌株H02,菌株H02经形态鉴定、16S rDNA鉴定为Halomonas属菌株,命名为Halomonas sp.H02。利用液相色谱与红外光谱特征峰分析的方法鉴定Halomonas sp.H02合成物质,确定其为Ectoine。经分泌性鉴定Halomonassp.H02为Ectoine非分泌型菌株。考察了Halomonas sp.H02的的生长及Ectoine发酵特性,其最适发酵培养基种类为MG改良培养基,最适碳源种类为谷氨酸钠,最适盐浓度为90 g/L;利用单因素实验考察了影响Ectoine合成的培养基各因素水平,并通过响应面法寻求最优的Ectoine发酵培养基,采用低渗透压冲击法制备Ectoine,所得制备率均值为94.0%。考察了辣根过氧化物酶(HRP)和漆酶两种污水处理常用酶的最适测定条件及Ectoine对极端环境下两种酶的的抗逆保护作用。结果表明:HRP酶的最适温度为30 ℃,最适pH为6,NaCl浓度对HRP酶活性具有明显抑制作用;漆酶对盐敏感,最适温度为40 ℃,最适pH为4。Ectoine对于高盐、极端温度、极端pH下酶活性的保护具有显著作用。将HRP、漆酶及其它具有降解石油、脱氮功能性的细胞粗酶干粉混合制备酶制剂,同时添加Ectoine作为抗逆助剂。结果表明:混合酶抗逆助剂对于模拟船舶含油污水(含油脱硫废液)具有显著处理效果,石油降解率最高达到52.5%,此时脱氮率为44.2%。
[Abstract]:High concentrations of ammonia nitrogen and petroleum alkanes are found in marine wastewater (ballast water, tank washing water, desulphurization waste) and so on. The biological purification and treatment of this kind of wastewater requires high salt adaptability, the characteristics and high efficiency of simultaneous degradation of ammonia nitrogen and petroleum alkanes. In this paper, the removal of nitrogen from simulated ship wastewater and the degradation of petroleum alkanes are studied by using the technology of purification and treatment of wastewater from wastewater with complex enzyme preparation assisted by anti-stress auxiliaries. A strain H02 capable of synthesizing Ectoine was isolated from the sediment of a salt pond in a salt field in Dalian. The strain H02 was identified as Halomonas genus by morphological identification of 16s rDNA and named Halomonas sp. H02. The synthetic compound of Halomonas sp.H02 was identified as Ectoine by liquid chromatography and infrared spectrum characteristic peak analysis. Halomonassp.H02 was identified as a non-secretory Ectoine strain by secretion. The growth of Halomonas sp.H02 and the characteristics of Ectoine fermentation were investigated. The optimum fermentation medium was MG improved medium and the most suitable carbon source was sodium glutamate. The optimum concentration of salt was 90 g / L, the factors affecting Ectoine synthesis were investigated by single factor experiment, and the optimal Ectoine fermentation medium was obtained by response surface method, and Ectoine was prepared by low osmotic pressure impact method. The average preparation rate was 94.0g 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1). The optimum conditions for the determination of horseradish peroxidase (HRP) and laccase (laccase) in wastewater treatment and the protective effect of Ectoine on the two enzymes in extreme environment were investigated. The results showed that the optimum temperature and pH of HRP enzyme were 30 鈩，
本文编号：2179549
[Abstract]:High concentrations of ammonia nitrogen and petroleum alkanes are found in marine wastewater (ballast water, tank washing water, desulphurization waste) and so on. The biological purification and treatment of this kind of wastewater requires high salt adaptability, the characteristics and high efficiency of simultaneous degradation of ammonia nitrogen and petroleum alkanes. In this paper, the removal of nitrogen from simulated ship wastewater and the degradation of petroleum alkanes are studied by using the technology of purification and treatment of wastewater from wastewater with complex enzyme preparation assisted by anti-stress auxiliaries. A strain H02 capable of synthesizing Ectoine was isolated from the sediment of a salt pond in a salt field in Dalian. The strain H02 was identified as Halomonas genus by morphological identification of 16s rDNA and named Halomonas sp. H02. The synthetic compound of Halomonas sp.H02 was identified as Ectoine by liquid chromatography and infrared spectrum characteristic peak analysis. Halomonassp.H02 was identified as a non-secretory Ectoine strain by secretion. The growth of Halomonas sp.H02 and the characteristics of Ectoine fermentation were investigated. The optimum fermentation medium was MG improved medium and the most suitable carbon source was sodium glutamate. The optimum concentration of salt was 90 g / L, the factors affecting Ectoine synthesis were investigated by single factor experiment, and the optimal Ectoine fermentation medium was obtained by response surface method, and Ectoine was prepared by low osmotic pressure impact method. The average preparation rate was 94.0g 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1). The optimum conditions for the determination of horseradish peroxidase (HRP) and laccase (laccase) in wastewater treatment and the protective effect of Ectoine on the two enzymes in extreme environment were investigated. The results showed that the optimum temperature and pH of HRP enzyme were 30 鈩，
本文编号：2179549
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